FlyBase Insertion Report: Dmel\P{lacW}kuz[k01403]

General Information

Symbol

Dmel\P{lacW}kuz^k01403

Species

D. melanogaster

Name

FlyBase ID

FBti0004828

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{lacW}

Uses

Affected gene(s)

Ecol\lacZ, kuz

Viability / fertility

Associated allele(s)

Ecol\lacZ^kuz-k01403, kuz^k01403

Stock availability

3 publicly available

LINE ID

l(2)k01403

Genomic Location

Chromosomal Location

2L ( 34C4 )

Sequence Location

2L:13,550,212..13,550,212 [+]

Target / Docking site

Genomic Maps

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Member of Large Scale Dataset(s)

Dataset

TI_set_P{lacW}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w^+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:13,550,212..13,550,212 [+]

(Sotillos et al., 1997, BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

34C4 (inferred by FlyBase from sequence location)

Cytological location (reported)

34C4-34C5 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ073300

(FlyBase, 1992-)

Inserted Element

Construct

P{lacW}

Location-dependent role

Size (Kb)

10.691

Component Allele(s)

w^+mC

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(BDGP Project Members, 1994-1999)

kuz

(Rooke et al., 1996)

Alleles and Phenotypes

Allele

Ecol\lacZ^kuz-k01403

Reference(s)

(BDGP Project Members, 1994-1999)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Allele

kuz^k01403

Reference(s)

(Koelzer and Klein, 2006, Koelzer and Klein, 2003, Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12, Spradling et al., 1999, Roch et al., 1998, Sotillos et al., 1997, Rooke et al., 1996, BDGP Project Members, 1994-1999)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)k01403

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (3)

Bloomington

10505

y¹ w^67c23; P{lacW}kuz^k01403/CyO

60690

w¹¹¹⁸; Df(2R)IR52a-d, P{lacW}kuz^k01403 PBac{RB5.WH5}IR52a-d/CyO

Kyoto

102204

y¹ w^67c23 ; P{lacW}kuz^k01403 / CyO

External Crossreferences and Linkouts ( 0 )

Comments

reported to be inserted 69 bp downstream of the 5' end of the longest kuz cDNA; GB:U60591 used as the longest cDNA

(Sotillos et al., 1997)

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

P{lacW}k01403

(Ashburner et al., 1999)

P{lacW}kuz^k01403

(Sotillos et al., 1997, Rooke et al., 1996, FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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