FlyBase Insertion Report: Dmel\P{PZ}ppl[00217]

General Information

Symbol

Dmel\P{PZ}ppl⁰⁰²¹⁷

Species

D. melanogaster

Name

FlyBase ID

FBti0004867

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)00217

Affected gene(s)

AcCoAS, Ecol\lacZ, ppl

Viability / fertility

Associated allele(s)

AcCoAS⁰⁰²¹⁷, Ecol\lacZ^ppl-00217, ppl⁰⁰²¹⁷

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 78C4 )

Sequence Location

3L:21,280,037..21,280,037 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3L:21,280,037..21,280,037 [-]

(Zinke et al., 1999)

Orientation

Cytological location (computed by FlyBase)

78C4 (inferred by FlyBase from sequence location)

Cytological location (reported)

78C3-78C4 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Genomic location previously reported as 3L:21273081..21273081 .

Sequence Data

Flanking sequence

G00740

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

AcCoAS

(FlyBase, 1996-)

Ecol\lacZ

(Zinke et al., 1999, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

ppl

(BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

AcCoAS⁰⁰²¹⁷

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^ppl-00217

Phenotypic Class Phenotype Manifest in Detailed Description

ppl⁰⁰²¹⁷

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

gastric caecum

(Meister and Braun, 1995.10)

embryonic/larval fat body

(Meister and Braun, 1995.10)

embryonic/larval midgut

(Meister and Braun, 1995.10)

adult stage

third segment of antenna

(Vosshall, 1998.8.10)

lamina

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)00217

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11490

w¹¹¹⁸; P{PZ}ppl⁰⁰²¹⁷ AcCoAS⁰⁰²¹⁷ ry⁵⁰⁶/TM3, Sb¹ Ser¹

External Crossreferences and Linkouts ( 0 )

Comments

Insertion occurs 451 bp downstream of ppl and 32 bp of one of the alternatively spliced transcripts of AcCoAS. Orientation of the insertion was not determined.

(Zinke et al., 1999)

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P1490

(Meister and Braun, 1995.10)

P{PZ}l(3)00217

(FlyBase, 1992-)

P{PZ}l(3)78Cb⁰⁰²¹⁷

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}ppl⁰⁰²¹⁷

(Zinke et al., 1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(3)78Cb⁰⁰²¹⁷

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000667

References (10)

Report Sections

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