FB2026_03 , released September 17, 2026
Insertion: Dmel\P{PZ}Sema2a03021
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General Information
Symbol
Dmel\P{PZ}Sema2a03021
Species
D. melanogaster
Name
FlyBase ID
FBti0005143
Feature type
transposable_element_insertion_site
Also Known As
P{PZ}Sema-2a03021
Description
Inserted Element
P{PZ}
LINE ID

l(2)03021

Affected gene(s)
Ecol\lacZ, Sema2a
Viability / fertility
Associated allele(s)
Ecol\lacZSema2a-03021, Sema2a03021
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
2R ( 53C6 )
Sequence Location
2R:16,517,270..16,517,274 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{PZ}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:16,517,270..16,517,274 [+]
(Matthes et al., 1995, Kolodkin et al., 1993)
Orientation
Cytological location (computed by FlyBase)
53C6 (inferred by FlyBase from sequence location)
Cytological location (reported)
53C6-53C10 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location

Reported orientation for this insertion may be in error; based on alignment and description in flanking sequence accession, orientation is 3' to 5' (minus).

(FlyBase, 1996-)

Genomic location previously reported as 2R:12404763..12404763 [+].

(BDGP Project Members, 1994-1999)
Sequence Data
Flanking sequence
AQ073276
(FlyBase, 1992-)
Inserted Element
Construct
P{PZ}
Size (Kb)

14.545

Component Allele(s)
l(3)87Df7.2
ry+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene
Ecol\lacZ
(Vosshall, 1998.8.10, Kolodkin et al., 1993)
Sema2a
(Kolodkin et al., 1993)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
larval neuron
(Kolodkin et al., 1993)
sense organ | anterior
(Kolodkin et al., 1993)
embryonic stage 14 -- 17
hypodermal muscle cell of metathorax | ventral
(Kolodkin et al., 1993)
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
gonad
(Kolodkin et al., 1993)
embryonic stage 10
epidermis | striped
(Kolodkin et al., 1993)
embryonic stage 15 -- 17
central nervous system | restricted
(Kolodkin et al., 1993)
adult stage
third segment of antenna
(Vosshall, 1998.8.10)
adult central complex
(Vosshall, 1998.8.10)
adult brain | restricted
(Vosshall, 1998.8.10)
Additional Information
Statement
Reference

In wandering third instar larva, no detectable β-gal staining.

(Meister and Braun, 1995.10)

Enhancer trap expression is firstdetected at embryonic stage 10 in weak epidermal stripes. It is also foundin the CNS starting in stage 15 in a restricted set of neurons. Beginningin stage 14, EcollacZ is expressed in a single ventral thoracic muscle fiberin the metathoracic segment. Expression occurs also in embryonic gonadsand anterior sense organs.

(Kolodkin et al., 1993)
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

l(2)03021

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Insertion site reported as after 2nd base of codon 32 in FBrf0066964. Insertion site reported as within codon 33 in FBrf0082330.

(Matthes et al., 1995, Kolodkin et al., 1993)
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
P1257
(Meister and Braun, 1995.10)
P{PZ}Sema-2a03021
(Matthes et al., 1995, Kolodkin et al., 1993, FlyBase, 1992-)
P{PZ}Sema2a03021
(FlyBase, 1992-)
P{PZ}l(2)0302103021
(FlyBase, 1992-)
P{PZ}sema-II03021
(Kolodkin et al., 1993, FlyBase, 1992-)
P{PZ}semaII03021
(Kolodkin et al., 1993, FlyBase, 1992-)
P{ry+t7.2=PZ}sema-II03021
(FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
  • FBti0000734
  • FBti0003558
References (16)