FlyBase Insertion Report: Dmel\P{PZ}flfl[01949]

General Information

Symbol

Dmel\P{PZ}flfl⁰¹⁹⁴⁹

Species

D. melanogaster

Name

FlyBase ID

FBti0005442

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)01949

Affected gene(s)

Ecol\lacZ, flfl, l(3)01949, omd

Viability / fertility

viable

Associated allele(s)

Ecol\lacZ^flfl-01949, flfl⁰¹⁹⁴⁹, omd⁰¹⁹⁴⁹

Stock availability

None publicly available

Genomic Location

Chromosomal Location

3R ( 87F10 )

Sequence Location

3R:13,684,434..13,684,434 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:13,684,434..13,684,434 [+]

(BDGP Project Members, 1994-1999)

Orientation

(Spradling, 1999.12.9, Spradling et al., 1999)

Cytological location (computed by FlyBase)

87F10 (inferred by FlyBase from sequence location)

Cytological location (reported)

87F10-87F11 (in situ hybridization reported)

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ026174

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Russell et al., 1998, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

flfl

(FlyBase, 1996-)

l(3)01949

(BDGP Project Members, 1994-1999)

omd

(FlyBase, 1996-)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^flfl-01949

Phenotypic Class Phenotype Manifest in Detailed Description

flfl⁰¹⁹⁴⁹

Phenotypic Class Phenotype Manifest in Detailed Description

omd⁰¹⁹⁴⁹

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

embryonic/larval midgut

(Meister and Braun, 1995.10)

adult stage

third segment of antenna

(Vosshall, 1998.8.10)

adult brain | restricted

(Vosshall, 1998.8.10)

adult central complex

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)01949

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Comments

Location 3R:9510156-9510157 confirmed by FlyBase alignment of dbGSS accession AQ026174 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

Insertion separable from lethal originally isolated on the same chromosome.

(Spradling et al., 1999)

No obvious phenotype.

(Spradling et al., 1999)

The lethality of the "l(3)01949" chromosome (caused by l(3)01949⁰¹⁹⁴⁹) is separable from the P{PZ}01949 insertion.

(Spradling, 1999.12.9)

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P{PZ}01949

(FlyBase, 1992-)

P{PZ}flfl⁰¹⁹⁴⁹

(FlyBase, 2005, FlyBase, 1992-)

P{PZ}l(3)01949⁰¹⁹⁴⁹

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(3)01949⁰¹⁹⁴⁹

(FlyBase, 1992-)

n(3)01949

(Spradling, 1999.12.9)

Name Synonyms

Secondary FlyBase IDs

FBti0000503

References (11)

Report Sections

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