FlyBase Insertion Report: Dmel\P{GAL4E69}puc[GAL4E69]

General Information

Symbol

Dmel\P{GAL4E69}puc^GAL4E69

Species

D. melanogaster

Name

FlyBase ID

FBti0025494

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{GAL4E69}

LINE ID

Affected gene(s)

puc, Scer\GAL4

Viability / fertility

Associated allele(s)

puc^GAL4E69, Scer\GAL4^puc-GAL4E69

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

Sequence Location

Target / Docking site

P{A92}puc^E69

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GAL4E69}

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

puc

(Adachi-Yamada, 2002)

Scer\GAL4

(Seisenbacher et al., 2011, Adachi-Yamada, 2002)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

puc^GAL4E69

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^puc-GAL4E69

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16

embryonic/larval head sense organ | subset

(Adachi-Yamada, 2002)

larval peripheral nervous system | restricted

(Adachi-Yamada, 2002)

adult stage | male

seminal vesicle

(Adachi-Yamada, 2002)

male accessory gland

(Adachi-Yamada, 2002)

Additional Information

Statement

Reference

Drives expression in JNK-activated cells; however not in embryonic leading edge cells.

(FlyBase Curators, 2017)

ScerGAL4^puc-GAL4E69 drives expression some sensory organ cells of the embryonic head, and in the embryonic peripheral nervous system. In the eye disc, ScerGAL4^puc-GAL4E69 drives expression posterior to the morphogenetic furrow; unlike endogenous puc, it also drives expression anterior to the morphogenetic furrow. In ommatidial clusters, ScerGAL4^puc-GAL4E69 drives expression in 1-2 cells per cluster. In the wing disc, ScerGAL4^puc-GAL4E69 drives expression in the wing pouch, in the presumptive scutellum, and in the peripodial membrane; similar expression patterns are observed in the leg and haltere discs.

(Adachi-Yamada, 2002)

ScerGAL4^puc-GAL4E69 does not drive expression in embryonic leading edge cells, where endogenous puc is expressed.

(Adachi-Yamada, 2002)

Marker for

Reflects expression of

puc

(Bloomington Drosophila Stock Center, 2017, Adachi-Yamada, 2002)

Reporter construct used in assay

Ecol\lacZ^UAS.cUa

(Adachi-Yamada, 2002)

Avic\GFP^{S65T.UAS.Tag:NLS(SV40-largeT)}

(Adachi-Yamada, 2002)