FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Insertion: Dmel\P{GawB}NP3612
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General Information
Symbol
Dmel\P{GawB}NP3612
Species
D. melanogaster
Name
FlyBase ID
FBti0035552
Feature type
transposable_element_insertion_site
Also Known As
NP3612
Description
Inserted Element
P{GawB}
LINE ID
Affected gene(s)
Scer\GAL4
Viability / fertility
Associated allele(s)
Scer\GAL4NP3612
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
X ( 3A2 )
Sequence Location
X:2,387,747..2,387,747 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{GawB}.NP
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)
Detailed Mapping Data
Chromosome (arm)

1

(Nakagawa et al., 2011)
Sequence Location
X:2,387,747..2,387,747 [+]
(FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)
3A2 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AG215692
(FlyBase, 1992-)
Inserted Element
Construct
P{GawB}
(Nakagawa et al., 2011)
Size (Kb)

11.279

Component Allele(s)
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene
Scer\GAL4
(Nakagawa et al., 2011, Higuchi et al., 2009)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 15
larval ventral nerve cord | restricted
(Higuchi et al., 2009)
embryonic brain | restricted
(Higuchi et al., 2009)
embryonic/larval midgut

Comment: fusion point

(Higuchi et al., 2009)
adult stage
adult optic lobe
(Higuchi et al., 2009)
adult antennal lobe
(Higuchi et al., 2009)
adult mushroom body
(Higuchi et al., 2009)
Additional Information
Statement
Reference

PsGEF is expressed in a subset of cells in the brain and ventral nerve cord as well as at the midgut fusion point in stage 15 embryos. It is also highly expressed in the optic lobes, antennal lobes, discrete neurons surrounding the antennal lobes, and mushroom bodies in the adult brain.

(Higuchi et al., 2009)
 
Marker for
Reflects expression of
PsGEF
(Higuchi et al., 2009)
Reporter construct used in assay
Mmus\Cd8aUAS.cLa.EGFP
(Higuchi et al., 2009)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Comments
Comments

Location X:2243902-2243903 refined by FlyBase alignment of dbGSS accession AG215692 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
NP3612
(Higuchi et al., 2009, Tanaka et al., 2007)
NP3612int
(Nakagawa et al., 2011)
P{GawB}NP3612
(FlyBase, 1992-)
P{GawB}NP3612-5-1
(FlyBase, 2005, FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
    References (7)