FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Insertion: Dmel\P{GawB}amnNP6013
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General Information
Symbol
Dmel\P{GawB}amnNP6013
Species
D. melanogaster
Name
FlyBase ID
FBti0036728
Feature type
transposable_element_insertion_site
Also Known As
NP6013
Description
Inserted Element
P{GawB}
LINE ID
Affected gene(s)
amn, Scer\GAL4
Viability / fertility
Associated allele(s)
amnNP6013, Scer\GAL4NP6013
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
X ( 19A2 )
Sequence Location
X:19,887,123..19,887,123 [-]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{GawB}.NP
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:19,887,123..19,887,123 [-]
(FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)
19A2 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AG217644
(FlyBase, 1992-)
Inserted Element
Construct
P{GawB}
Size (Kb)

11.279

Component Allele(s)
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene
amn
(FlyBase Curators, 2008-)
Scer\GAL4
(Hasegawa et al., 2011, Pauls et al., 2010, Tanaka et al., 2008)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
larval neuron | subset of medulla anlage

Comment: inner region

(Hasegawa et al., 2011)
late third instar larval stage
neuron | subset of medulla
(Hasegawa et al., 2013)
prepupal stage P1
neuron | subset of medulla
(Hasegawa et al., 2011)
adult stage
mushroom body dorsal paired medial cell
(Flybrain Neuron Database et al., 2018.6.28, Tanaka et al., 2008)
adult mushroom body alpha-lobe
(Tanaka et al., 2008)
adult mushroom body beta-lobe
(Tanaka et al., 2008)
pedunculus of adult mushroom body
(Tanaka et al., 2008)
adult dopaminergic PAM neuron
(Tanaka et al., 2008)
mushroom body gamma lobe slice 4
(Tanaka et al., 2008)
mushroom body beta' anterior-posterior layer
(Tanaka et al., 2008)
mushroom body beta' lobe slice 1
(Tanaka et al., 2008)
mushroom body beta' lobe slice 2
(Tanaka et al., 2008)
mushroom body beta' middle layer
(Tanaka et al., 2008)
neuron | subset of medulla
(Hasegawa et al., 2011)
lamina wide-field 2 neuron
(Hasegawa et al., 2011)
adult mushroom body gamma-lobe
(Tanaka et al., 2008)
adult mushroom body beta'-lobe
(Tanaka et al., 2008)
adult mushroom body alpha'-lobe
(Tanaka et al., 2008)
Additional Information
Statement
Reference

In third instar larva, ScerGAL4amn-NP6013 drives expression in the putative medullary intrinsic neuron Mi1.

(Hasegawa et al., 2013)

P{GawB}amnNP6013 is expressed in the inner region of the medulla anlage of third instar larva. At pupariation and in adults P{GawB}amnNP6013 is expressed in a subset of neurons in the medulla. In adults, it labels the lamina wide-field cell Lawf2.

(Hasegawa et al., 2011)
 
Marker for
Reflects expression of
amn
(Hasegawa et al., 2013, Hasegawa et al., 2011, Tanaka et al., 2008)
Reporter construct used in assay
Mmus\Cd8aUAS.cLa.EGFP
(Hasegawa et al., 2013, Tanaka et al., 2008)
Avic\GFPS65T.UAS
(Tanaka et al., 2008)
Mmus\Cd8aFRT.UAS.GFP
(Hasegawa et al., 2011)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Comments
Comments

Location X:19721059-19721060 refined by FlyBase alignment of dbGSS accession AG217644 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
NP6013
(Flybrain Neuron Database et al., 2018.6.28, Hasegawa et al., 2011, Pauls et al., 2010, Tanaka et al., 2008)
NP6013-Gal4
(Hasegawa et al., 2013)
P{GawB}NP6013
(FlyBase, 1992-)
P{GawB}NP6013-5-1
(FlyBase, 2005, FlyBase, 1992-)
P{GawB}amnNP6013
Name Synonyms
Secondary FlyBase IDs
    References (9)