FlyBase Insertion Report: Dmel\P{GawB}NP7088

General Information

Symbol

Dmel\P{GawB}NP7088

Species

D. melanogaster

Name

FlyBase ID

FBti0037217

Feature type

transposable_element_insertion_site

Also Known As

NP7088-GAL4, NP7088

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

Associated allele(s)

Scer\GAL4^NP7088

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 49D7 )

Sequence Location

2R:12,880,289..12,880,289 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:12,880,289..12,880,289 [+]

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

49D7 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AG217128

(FlyBase, 1992-)

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Burke et al., 2012, Schneider et al., 2012, Pauls et al., 2010, Busch et al., 2009, Tanaka et al., 2008)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^NP7088

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

octopaminergic neuron | subset

(Fei et al., 2018)

adult stage

adult brain | restricted

(Matsuo et al., 2016, Busch et al., 2009)

adult octopaminergic subesophageal VM neuron

(Busch et al., 2009)

octopaminergic AL2 neuron

(Busch et al., 2009)

mushroom body beta' lobe slice 1 | faint

(Tanaka et al., 2008)

mushroom body beta' lobe slice 2 | faint

(Tanaka et al., 2008)

calyx of adult mushroom body

(Tanaka et al., 2008)

adult mushroom body dorsal accessory calyx

(Tanaka et al., 2008)

adult mushroom body gamma-lobe

(Tanaka et al., 2008)

octopaminergic VUMa2 neuron

(Burke et al., 2012)

octopaminergic VPM3 neuron

(Burke et al., 2012)

octopaminergic VPM4 neuron

(Burke et al., 2012)

antennal mechanosensory and motor center AMMC Bi3 neuron

(Matsuo et al., 2016)

antennal mechanosensory and motor center AMMC Bi13 neuron

(Matsuo et al., 2016)

antennal mechanosensory and motor center AMMC Bi16 neuron

(Matsuo et al., 2016)

descending neuron of the gnathal ganglion DNg24

(Matsuo et al., 2016)

larval VUM neuron

(Flybrain Neuron Database et al., 2018.6.28)

alpha/beta posterior Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

alpha/beta core Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

Additional Information

Statement

Reference

ScerGAL4^NP7088 drives expression in a pattern similar to that of ScerGAL4^Tdc2.PS, labeling most of the ScerGAL4^Tdc2.PS-expressing neurons in the thoracic and abdominal segments of the larval ventral nerve cord, though not those in the brain and subesophageal ganglion.

(Fei et al., 2018)

ScerGAL4^NP7088 drives expression in several clusters of cells in the adult, in a total of 333 cells per hemisphere. These clusters include the octopaminergic neurons VM (34 cells) and AL2 (11 cells). In addition, ScerGAL4^NP7088 labels cells in the middle inferior lateral protocerebrum (MIL cluster, 97 cells), middle superior medial protocerebrum (MSM cluster, 12 cells), posterior lateral protocerebrum (PL cluster, 19 cells), and in the lateral subesophageal ganglion (SOG cluster, 18 cells).

(Busch et al., 2009)

Marker for

Reflects expression of

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Busch et al., 2009, Tanaka et al., 2008)

Avic\GFP^S65T.UAS

(Tanaka et al., 2008)

Mmus\Cd8a^UAS.mCherry

(Burke et al., 2012)

Disc\RFP^S197Y.UASp

(Matsuo et al., 2016)

Mmus\Cd8a^FRT.UAS.GFP

(Matsuo et al., 2016)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Fei et al., 2018)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Kyoto

105362

y^* w^*; P{GawB}NP7088 / CyO, P{UAS-lacZ.UW14}UW14

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AG217128

Comments

Location 2R:8396195-8396196 refined by FlyBase alignment of dbGSS accession AG217128 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

NP7088

(Flybrain Neuron Database et al., 2018.6.28, Matsuo et al., 2016, Bouzaiane et al., 2015, Schneider et al., 2012, Pauls et al., 2010, Busch et al., 2009, Tanaka et al., 2008)

NP7088-GAL4

(Hermanns et al., 2022, Damrau et al., 2017, Burke et al., 2012)

NP7088-Gal4

(Ruppert et al., 2026, Fei et al., 2018)

P{GawB}NP7088

(FlyBase, 1992-)

P{GawB}NP7088-5-1

(FlyBase, 2005, FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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