A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.
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ScerGAL4NP3212 drives expression in mushroom body extrinsic neurons, including mushroom body medial lobe arborizing neurons 4, 5, and 6, that innervate mushroom body gamma lobe slices 3 and 5, and mushroom body beta' lobe slice 2.
Beta' lobe expression has only been mapped for medial lobe arborizing neuron 4, as the arborization patterns of medial lobe arborizing neuron 5 and 6 have not yet been fully characterized. Gamma lobe segment 5 expression is found only with medial lobe arborizing neuron 6.
