A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.
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robo3 reporter is expressed specifically and transiently in photoreceptor R8 growth cones. It is expressed in third instar larval R8 growth cones as they enter the optic lobe, displays a graded pattern of staining with decreased staining in axons extending further toward their final layer, and is downregulated shortly after the R8 axons enter the medulla neuropil.
