The construct was used as a reporter of JNK pathway activity. Reporter expression during the larval and pupal stages is dynamic, asymmetrical, and genitalia specific. Levels are higher and more widespread in females. In both sexes in all stages analyzed, expression is observed in some anterior segment A8 cells and in a subset of en A10 cells that probably correspond to stalk cells. In males in late third instar, apart from the stalk cells, expression is seen in cells at the A8 A/P border. In females at the same time, expression is in the stalks and in scattered en and anterior cells all over the disc but expression is more concentrated around the A8 A/P border. In the prepupal stage, the pattern is particularly sexually dimorphic in the genital primordia. In the male A9 genitalia primordium, random expression is observed along with a continuous band bordering the left and right halves of the bilateral region. In the female A8 genitalia primordium, expression is confined to the lateral left-right regions and to scattered cells at the bilateral region. The reporter is co-expressed with Decay.