The insertion site was reported to be 32 bases from the subdued translational start site.
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ScerGAL4subdued-c240 drives expression in class IV dendritic arborizing neurons involved in nociception. In addition, expression is observed in some cells of the larval and adult central nervous system. Some non-neural cells are labelled in the proventriculus, oviduct, Malpighian tubule and spermathecum.
The orientation of the insertion was not reported.