FlyBase Insertion Report: Dmel\Mi{Trojan-p65AD.2}Gad1[MI09277-Tp65AD.2]

General Information

Symbol

Dmel\Mi{Trojan-p65AD.2}Gad1^{MI09277-Tp65AD.2}

Species

D. melanogaster

Name

FlyBase ID

FBti0168163

Feature type

transposable_element_insertion_site

Also Known As

Gad1-AD, Gad1^MI09277-p65AD, GAD1-p65.AD

Description

Inserted Element

Mi{Trojan-p65AD.2}

LINE ID

Affected gene(s)

Gad1

Viability / fertility

Associated allele(s)

Gad1^{MI09277-Tp65AD.2}, Hsap\RELA^{Gad1-MI09277-Tp65AD.2}

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 64A5 )

Sequence Location

Target / Docking site

Mi{MIC}Gad1^MI09277

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

64A5 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

Mi{Trojan-p65AD.2}

(Diao et al., 2015)

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

Gad1

(Diao et al., 2016, Diao et al., 2015)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Gad1^{MI09277-Tp65AD.2}

Phenotypic Class Phenotype Manifest in Detailed Description

Hsap\RELA^{Gad1-MI09277-Tp65AD.2}

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

adult GABAergic doublesex AbN (female) neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

(Pavlou et al., 2016)

adult stage | male

adult GABAergic doublesex AbN (male) neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

(Pavlou et al., 2016)

adult doublesex SN (male) neuron | subset

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

(Pavlou et al., 2016)

adult fruitless pIP-e (male) neuron | subset

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

(Pavlou et al., 2016)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Replacement descendant of (target)

Mi{MIC}Gad1^MI09277

(Diao et al., 2015)

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

60322

y¹ w^*; Mi{Trojan-p65AD.2}Gad1^{MI09277-Tp65AD.2}/TM6B, Tb¹

External Crossreferences and Linkouts ( 0 )

Comments

phiC31-mediated recombination has been used to replace the attP cassette of the Mi{MIC} element with the intron phase 2 "Trojan p65AD" cassette from the pBS-KS-attB2-SA(2)-T2A-p65AD-Hsp70 plasmid.

(Diao et al., 2015)

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

GAD-GAL4-AD

(Syed et al., 2026)

GAD1-AD

(Guo et al., 2026)

GAD1-p65.AD

(Erginkaya et al., 2025)

GAD1^MI09277-p65AD

(Wei et al., 2020)

Gad1-AD

(Jones et al., 2025, Jung et al., 2020)

Gad1-Trojan-p65AD

(Collie et al., 2026)

Gad1^AD

(Babski et al., 2019)

Gad1^MI09277-p65AD

(Diao et al., 2016, Diao et al., 2015)

Gad1^p65-AD

(Pavlou et al., 2016)

Gad1p65AD

(Imoto et al., 2024)

Mi{Trojan-p65AD.2}Gad1^{MI09277-Tp65AD.2}

gad1-AD

(Lacin et al., 2019)

gad1-p65.AD

(Soffers et al., 2025)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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