FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Recombinant Construct: P{Ubi-GFP.nls}
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General Information
Symbol
P{Ubi-GFP.nls}
FlyBase ID
FBtp0001356
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPUbi-p63E.Tag:NLS(Unk)
Also Known As
ubi-nlsGFP, nlsGFP, Ubi-GFPnls, NLS-GFP, ubi-nls GFP
Size
Expression Data
GFP reporter
Associated insertion(s)
8 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Avic\GFPUbi-p63E.Tag:NLS(Unk)
Transgenic product class
Component
Use(s)
Regulatory region(s)
Ubi-p63E
Encoded product / tool
GFP
Tagged with
Tag:NLS(Unk)
Description

1980bp of Ubi-p63E promoter (and first four amino acids) directs the expression of a 9 amino acid nuclear localization sequence fused to 40 amino acids of Ecol\lacZ (from its N-terminus) fused to full length GFP followed by Hsp27 polyadenylation sequence.

(Davis et al., 1995)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
organism | ubiquitous
(Davis et al., 1995)
(Davis et al., 1995)
Additional Information
Statement
Reference

In early embryo, used as nuclear marker for identification of cells within mitotic domains.

(Namba and Minden, 1999)

Shows highly fluorescent green nuclei and less fluorescent cytoplasm. (Fusion protein contains nuclear localization signal.) There is a long delay between zygotic expression and appearance of fluorescence.

(Davis et al., 1995)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{Ubi-GFP.nls}
(Davis et al., 1995)
descendant(s)
Comments

report comment: The Ubi::lacZ::GFP fusion protein is highly fluorescent when maternally supplied, but there is a long delay between zygotic expression and appearance of fluorescence.

(Davis et al., 1995)
Stocks (12)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (20)
References (35)