FLP-induced recombination between the FRT sites of P{RS3} leaves the 3' exon of w+mW.FRT.hs flanked on its 5' end by a FRT site oriented divergently with respect to the direction of w transcription.
FLP-induced recombination between the FRT sites of P{RS3} leaves the 3' exon of w+mW.FRT.hs flanked on its 5' end by a FRT site oriented divergently with respect to the direction of w transcription.
"Remnant" element generated by excision of FRT-flanked sequences from P{RS3} upon exposure to FLPase. Specific insertions may be used in conjunction with reciprocal insertions of P{RS5r} to induce chromosomal rearrangements defined by the original sites of the insertions. Some rearrangement classes can be selected by regeneration of mini-white marker in P{3'.RS5+3.3'}.
construct_comment: Target for FLP1-recombinase-mediated targeted integration of FRT-bounded cassettes.
Carries 3' portion of w+mW.FRT.hs.
"Remnant" element generated by excision of FRT-flanked sequences from P{RS3} upon exposure to FLPase. Specific insertions may be used in conjunction with reciprocal insertions of P{RS5r} to induce chromosomal rearrangements defined by the original sites of the insertions. Some rearrangement classes can be selected by regeneration of mini-white marker in P{3'.RS5+3.3'}.