FB2026_02 , released June 18, 2026
Recombinant Construct: P{UAS-RhoL.N25}
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General Information
Symbol
P{UAS-RhoL.N25}
FlyBase ID
FBtp0001697
Feature type
transgenic_transposable_element
Component Allele(s)
RhoLN25.UAS
Also Known As
UAS-RhoLN25
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele RhoLN25.UAS
Transgenic product class
dominant_negative_variant
(Murphy and Montell, 1996)
missense_variant
(Murphy and Montell, 1996)
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
RhoL
Description

UASt regulatory sequences drive expression of RhoL that carries the amino acid replacement T25N. This is expected to result in a dominant-negative protein (the analagous change in Ras causes preferential binding of GDP compared to GTP).

(Murphy and Montell, 1996)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{UAST}
(Murphy and Montell, 1996)
descendant(s)
Comments

construct_comment: Progenitors are those of the plasmid, pP{UAS-RhoL.N25}.

(Murphy and Montell, 1996)
Stocks (2)
Stocks containing insertion(s) of this recombinant construct
Bloomington
4849
w1118; P{UAS-RhoL.N25}AM1/CyO
Kyoto
107907
w1118; P{UAS-RhoL.N25}AM1/CyO
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
P{UAS-RhoL.N25}
(FlyBase, 1996-)
P{w+mC=hs-RhoL.N25}
(Paladi and Tepass, 2004)
RhoN25
(Emoto et al., 2004)
UAS-DrhoL(N25)
(Shuai et al., 2010)
UAS-RhoLN25
(Paladi and Tepass, 2004, Kunwar et al., 2003)
UAS::RhoLN25
(van Impel et al., 2009)
UAST-RhoLN25
(Murphy and Montell, 1996)
Secondary FlyBase IDs
    References (9)