FB2026_03 , released September 17, 2026
Recombinant Construct: P{ChAT.7.4kb-lacZ}
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General Information
Symbol
P{ChAT.7.4kb-lacZ}
FlyBase ID
FBtp0004021
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZChAT.7.4P
Size
Expression Data
lacZ reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Ecol\lacZChAT.7.4P
Transgenic product class
Component
Use(s)
Regulatory region(s)
ChAT_ChAT-7.4kb
Encoded product / tool
lacZ
Description

7.4kb BamHI fragment containing ChAT 5' flanking region, transcription start site and 391bp of non coding sequence present in the first exon drives expression of Ecol\lacZC4.Adh.

(Kitamoto et al., 1992)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
accessory pharyngeal nerve
(Kitamoto et al., 1995)
cholinergic neuron
(Kitamoto et al., 1995)
supraesophageal ganglion
(Kitamoto et al., 1995)
sensory neuron of antenna
(Kitamoto et al., 1992)
leg sensillum | subset
(Kitamoto et al., 1995)
sensory neuron of adult labral sense organ
(Kitamoto et al., 1995)
sensory neuron of wing vein L1
(Kitamoto et al., 1995)
gnathal ganglion
(Kitamoto et al., 1995)
adult labral sense organ
(Kitamoto et al., 1995)
male terminalia sensillum | subset
(Kitamoto et al., 1995)
adult optic lobe
(Kitamoto et al., 1992)
wing vein L1 | restricted
(Kitamoto et al., 1995)
adult central complex
(Kitamoto et al., 1992)
sensory neuron of antennal sense organ
(Kitamoto et al., 1995)
antennal sense organ
(Kitamoto et al., 1995)
sensory neuron of male terminalia sensillum
(Kitamoto et al., 1995)
sensory neuron of leg sensillum
(Kitamoto et al., 1995)
adult ventral nerve cord
(Kitamoto et al., 1995)
adult labellum sensillum
(Kitamoto et al., 1995)
antenna
(Kitamoto et al., 1992)
adult hypocerebral ganglion
(Kitamoto et al., 1995)
sensory neuron of adult labellum sensillum
(Kitamoto et al., 1995)
sensory neuron of maxillary palp sense organ
(Kitamoto et al., 1995)
maxillary palp sense organ
(Kitamoto et al., 1995)
adult antennal lobe
(Kitamoto et al., 1992)
Additional Information
Statement
Reference

In adult optic lobe, appears to be expressed in the same cells as Cha protein, but in the entire cell (including soma), whereas Cha protein is detected only at the nerve endings in the neuropil (thus expression appears to be more extensive).

(Kitamoto et al., 1992)
 
Marker for
cholinergic neuron
(Kitamoto et al., 1995)
Reflects expression of
ChAT
(Kitamoto et al., 1995, Kitamoto et al., 1992)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
7.4kb-ChAT/lacZ
(Yasuyama and Salvaterra, 1999)
P{ChAT.7.4kb-lacZ}
(FlyBase, 1996-)
P{Cha.7.4kb-lacZ}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (10)