2.2dis element, located 2.5kb upstream from the start of transcription, is fused a non-heat-inducible fragment of the Hsp70 promoter fused to codon 8 of lacZ.
The 2.2 kb upstream fragment was sufficient, when coupled either with a Hsp70 promoter or a 1.2 kb ci promoter, to drive EcollacZ expression in embryos and imaginal discs in a wild-type ci expression pattern. EcollacZ expression is seen in a segmentally repeated pattern from embryonic gastrulation through germ band extension, and in the anterior compartment of imaginal discs.