lacZ coding region fused to a nuclear localisation sequence is expressed under the control of an Hsp70 promoter.
Construct: An I-element (derived from the wIR3 I-element) marked with Ecol\lacZRT gene inserted in place of a 2.6kb fragment deleted from the second open reading frame. The deletion encompasses the previously identified reverse transcriptase, RNase H and 3' zinc finger domains.
Construct: An I-element (derived from the wIR3 I-element) marked with Ecol\lacZRT gene inserted in place of a 2.6kb fragment deleted from the second open reading frame. The deletion encompasses the previously identified reverse transcriptase, RNase H and 3' zinc finger domains.