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General Information
Symbol
P{islH-tau-myc}
FlyBase ID
FBtp0007840
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
islH-tau-myc, islet-tau-myc, isl-H-tau-myc
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Component allele Btau\MAPTisl.H.Tag:MYC
Product class / Tool use(s)
Description

A 7kb genomic fragment located 6 to 13kb upstream of the tup putative transcriptional start site is fused upstream of a minimal Hsp70 promoter driving expression of bovine Btau\MAPT tagged with six copies of Tag:MYC.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
molecular
tag
Component Alleles
Molecular data

A 7kb genomic fragment located 6 to 13kb upstream of the tup putative transcriptional start site is fused upstream of a minimal Hsp70 promoter driving expression of bovine Btau\MAPT tagged with six copies of Tag:MYC.

Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Btau\MAPTisl.H.T:Hsap\MYC drives expression in a subset of ventral motor neurons which exit the ventral nerve cord via the ISNb nerve branch. There is co-expression with Oli protein.

Reporter construct studies indicate that Lim3 is co-expressed with tup in a subset of motor neurons. For the intersegmental nerve, all the ISNb-specific motor neurons express both Lim3 and tup, while those projecting in ISNd only express tup. Lim3 is not co-expressed with tup or ap in interneurons. Motor neurons co-expressing tup and Lim3 include motor neurons RP1, RP3, RP4, RP5, the contralateral V motor neuron which innervates muscle 12, and MN28 and MN14/30 which innervate muscles 28 and 14 plus 30, respectively. The tup-expressing motor neurons projecting in ISNd innervate muscles 15-17.

Reporter expression is not detected in neuroblasts but in postmitotic neurons, in a subset of motor neurons and interneurons. The subset of motor neurons expressing the reporter innervate muscles located ventrally in the body wall, and include RP1, RP3 and RP4. Reporter expression is detected in 3 morphologically different classes of interneurons. Double staining for ap and tup reporters reveal that they are expressed in nonoverlapping subsets of neurons that project axons along different pathways.

 
Marker for
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
Reported As
Secondary FlyBase IDs
    References (15)