FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{btl(FRT.y+)GAL4}
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General Information
Symbol
P{btl(FRT.y+)GAL4}
FlyBase ID
FBtp0020129
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\GAL4btl.B123.FRT
Also Known As
btl>y+>GAL4
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
y+t7.7
Component allele Scer\GAL4btl.B123.FRT
Transgenic product class
Component
Use(s)
Regulatory region(s)
btl
Encoded product / tool
GAL4
Also carries
FRT
Description

The "B123" btl upstream fragment is separated from sequence encoding a GAL4 driver by an FRT cassette containing y+t7.7.

(Ribeiro et al., 2004)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
tracheal section

Comment: heat-shock dependent

(Ribeiro et al., 2004)
Additional Information
Statement
Reference

The Gal4 coding sequence was separated from the btl enhancer by a flip-out cassette containing the sequence of y (including transcriptional stop signals) flanked by FRT recombination sites to avoid constitutive expression of the transcriptional activator Gal4 in the entire tracheal system. Induction of the FLP recombinase with a mild heat shock resulted in the removal of the y cassette in individual cells, allowing for the transcription of the Gal4 gene in a small fraction of btl-Gal4 positive cells.

(Ribeiro et al., 2004)
 
Marker for
Reflects expression of
btl
(Ribeiro et al., 2004)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
P{btl(FRT.y+)GAL4}
(FlyBase, 1996-)
btl>y+>GAL4
(Öztürk-Çolak et al., 2016, Warrington et al., 2013)
btl>y+>Gal4
(Du et al., 2022)
btl>y+>Gal4
(Du et al., 2018)
btlenhancer > y+ > Gal4
(Caussinus et al., 2008)
btlenhancer>y+>Gal4
(Ribeiro et al., 2004)
Secondary FlyBase IDs
    References (8)