FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Recombinant Construct: P{ppk-EGFP.1}
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General Information
Symbol
P{ppk-EGFP.1}
FlyBase ID
FBtp0041053
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
ppk-EGFP, ppk-GFP, ppk-EGFP5, pickpocket-EGFP
Size
Expression Data
GFP reporter
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPEGFP.ppk.1
Transgenic product class
Component
Use(s)
Regulatory region(s)
Encoded product / tool
Description

A ~1kb fragment from the ppk enhancer region fused to a minimal Hsp70 promoter drives expression of EGFP.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

AvicGFP1.E.ppk drives expression in 4 clusters of neurons in the adult female genital tract: one cluster of 2 neurons in the lateral oviduct (with one neuron co-localising with ScerGAL4dsx.KI and the other with EcollexAfru.P1.T:HsimVP16) and three clusters in the uterus. An anterior one with 3 neurons (which also express ScerGAL4dsx.KI) and one more posterior with 7 neurons which co-express ScerGAL4dsx.KI, with 3 neurons of those also expressing EcollexAfru.P1.T:HsimVP16. The most posterior cluster in the uterus is composed of 5 neurons.

Signal is observed in the axonal terminal branches of of class IV dendritic arborizing (C4da) neurons in the ventral nerve cord as a ladder-like pattern with a longitudinal fascicle on each side and a commissural fascicle in every segment.

AvicGFPEGFP.ppk.1 is expresssed in 2-3 ScerGAL4fru.P1.D positive fruitless expressing neurons either side of the uterus.

In females, AvicGFP1.E.ppk drives expression in 2 neurons on each lateral oviduct and 15 neurons on each side of the uterus, organised in 3 clusters. Eight of these reproductive tract neurons labeled by AvicGFP1.E.ppk co-express ScerGAL4fru.P1.D. In males, AvicGFP1.E.ppk labels 7 neurons in the ejaculatory duct, with two of these co-expressing ScerGAL4fru.P1.D.

This reporter reproduces the expression of the ppk gene in Type IV dendritic aborising neurons. There is also a low level of expression in some Type III neurons.

 
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
References (27)