Open Close
General Information
Symbol
P{ppk-EGFP.1}
FlyBase ID
FBtp0041053
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
ppk-EGFP, ppk-GFP, ppk-EGFP5, pickpocket-EGFP
Size
Expression Data
GFP reporter
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
Construct components
Component allele Avic\GFPEGFP.ppk.1
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Description

A ~1kb fragment from the ppk enhancer region fused to a minimal Hsp70 promoter drives expression of EGFP.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
reporter
gene
Component Alleles
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Avic\GFPEGFP.ppk.1 drives expression in 4 clusters of neurons in the adult female genital tract: one cluster of 2 neurons in the lateral oviduct (with one neuron co-localising with Scer\GAL4dsx.KI and the other with Ecol\lexAfru.P1.T:Hsim\VP16) and three clusters in the uterus. An anterior one with 3 neurons (which also express Scer\GAL4dsx.KI) and one more posterior with 7 neurons which co-express Scer\GAL4dsx.KI, with 3 neurons of those also expressing Ecol\lexAfru.P1.T:Hsim\VP16. The most posterior cluster in the uterus is composed of 5 neurons.

Signal is observed in the axonal terminal branches of of class IV dendritic arborizing (C4da) neurons in the ventral nerve cord as a ladder-like pattern with a longitudinal fascicle on each side and a commissural fascicle in every segment.

Avic\GFPEGFP.ppk.1 is expresssed in 2-3 Scer\GAL4fru.P1.D positive fruitless expressing neurons either side of the uterus.

In females, Avic\GFPEGFP.ppk.1 drives expression in 2 neurons on each lateral oviduct and 15 neurons on each side of the uterus, organised in 3 clusters. Eight of these reproductive tract neurons labeled by Avic\GFPEGFP.ppk.1 co-express Scer\GAL4fru.P1.D. In males, Avic\GFPEGFP.ppk.1 labels 7 neurons in the ejaculatory duct, with two of these co-expressing Scer\GAL4fru.P1.D.

This reporter reproduces the expression of the ppk gene in Type IV dendritic aborising neurons. There is also a low level of expression in some Type III neurons.

 
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
References (26)