FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{Prosα6T-EGFP.M}
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General Information
Symbol
P{Prosα6T-EGFP.M}
FlyBase ID
FBtp0050456
Feature type
transgenic_transposable_element
Component Allele(s)
Prosα6TEGFP
Also Known As
Prosα6T-GFP
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mW.hs
Component allele Prosα6TEGFP
Transgenic product class
wild_type
(Ma et al., 2002)
Component
Use(s)
Regulatory region(s)
Prosα6T
Encoded product / tool
Prosα6T
Tagged with
EGFP
Description

Contains the Prosα6T gene, with 0.8kb of upstream sequences and 1.1kb of downstream sequences. The Prosα6T open reading frame is tagged at the C-terminal end with EGFP.

(Ma et al., 2002)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
spermatogenesis
spermatid
(Ma et al., 2002)
spermatozoon
(Ma et al., 2002)
epitope tag
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | male
spermatid cyst
(Zhong and Belote, 2007)
elongation stage spermatid
(Zhong and Belote, 2007)
individualization stage spermatid
(Zhong and Belote, 2007)
spermatozoon
(Zhong and Belote, 2007)
Additional Information
Statement
Reference

Prosα6T-GFP becomes prominent in the cytoplasm and nuclei of 64-cell spermatid cysts. Signal persists in the elongated and condensed spermatid nuclei and also in spermatid tail bundles. It is seen in mature sperm heads and tails. Prosα6T-GFP is also found in speckles near the individualization complex.

(Zhong and Belote, 2007)

Prosα6TT:AvicGFP-EGFP is expressed only in the testis, and is not expressed until after the second meiotic division. Expression continues through then end of spermatogenesis. Prosα6TT:AvicGFP-EGFP can be observed in the tails of mature sperm cells.

(Ma et al., 2002)
 
Marker for
Reflects expression of
Prosα6T
(Zhong and Belote, 2007, Ma et al., 2002)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Alpha6T-GFP
(Bader et al., 2010)
Prosα6T-GFP
(Yun et al., 2008, Zhong and Belote, 2007)
P{Prosα6T-EGFP.M}
(FlyBase, 1992-)
pW8/Prosα6T-GFP
(Ma et al., 2002)
Secondary FlyBase IDs
    References (6)