FlyBase Recombinant Construct Report: P{Gr33a-GAL4.4.7}

Expression of UAS- mCD8::GFP via ScerGAL4^Gr33a.4.7 confirmed that it is expressed in 12 GRN pairs. These 12 pairs are suggested to represent the entire set of larval bitter receptor neurons. They are: (i) two pairs B1 and B2 in the dorsolateral group of sensilla of the TO, (ii) four pairs C1-C4 in the distal group of TO sensilla, (iii) two pairs D1 and D2 in the DPS (epiphysis), (iv) two pairs E1 and E2 in the VPS (hypophysis), and (v) two pairs F1 and F2 in the PPS (hypopharyngeal organ).

(Apostolopoulou et al., 2016)

ScerGAL4^Gr33a.4.7 drives expression in adult abdominal multidendritic neurons with with dorsal processes extending along the left-right axis, as well as in multidendritic neurons with lateral processes extending anteroposteriorly; these major processes have several protruding dendrites. Expression is also observed in projections to the abdominal ganglion.

(Park and Kwon, 2011)

ScerGAL4^Gr33a.4.7 is expressed in the gustatory neurons of all of the bitter-sensitive short labellar taste bristles, and the gustatory neurons of all of the intermediate labellar taste bristles.

(Weiss et al., 2011)

ScerGAL4^Gr33a.4.7 is expressed in all of the short and intermediate bitter-sensitive sensilla of the labellum.

(Weiss et al., 2011, Weiss et al., 2011)

ScerGAL4^Gr33a.4.7 drives expression in 6 gustatory receptor neurons of the larval terminal organ, two of these having its cell body in the dorsal organ ganglion (TO-dorsolateral group). All 6 neurons co-express ScerGAL4^Gr66a.1.8. One of the neurons of the TO-dorsolateral group also expresses ScerGAL4^Gr10a.3.7 and ScerGAL4^Gr32a.3.7.PW. The other terminal organ neurons express different combinations of drivers. The first neuron expresses ScerGAL4^Gr32a.3.7.PW, ScerGAL4^{Gr28b.e.4.245}, ScerGAL4^Gr39a.a.3.4, ScerGAL4^Gr39a.b.1, ScerGAL4^Gr59d.4.1, ScerGAL4^Gr9a.6.3, ScerGAL4^Gr22e.2.5, ScerGAL4^Gr22a.4.2, ScerGAL4^Gr28b.a.3, ScerGAL4^Gr36b.0.9, ScerGAL4^Gr36c.2.5, ScerGAL4^Gr58b.1, ScerGAL4^Gr59a.1.5, ScerGAL4^Gr59c.5.5 and ScerGAL4^Gr93b.5.3. The second neuron co-expresses ScerGAL4^Gr39a.a.3.4, ScerGAL4^Gr57a.6, ScerGAL4^Gr59d.4.1, ScerGAL4^Gr47b.2 and ScerGAL4^Gr94a.3.7. The third neuron co-expresses ScerGAL4^Gr57a.6 and ScerGAL4^Gr97a.1. The fourth neuron expresses ScerGAL4^Gr39a.b.1 and ScerGAL4^Gr59d.4.1. Expression of ScerGAL4^Gr33a.4.7 is also found in two neurons of the dorsal pharyngeal sense organ (DPS or epiphysis), two neurons of the posterior pharyngeal sense organ (PPS or hypopharyngeal sense organ) and in two neurons of the ventral pharyngeal sense organ (VPS or hypophysis).

(Kwon et al., 2011)

Marker for

bitter-sensing neuron

(Israel et al., 2022)

Reflects expression of

Gr33a

(Israel et al., 2022, Apostolopoulou et al., 2016, Ling et al., 2014, Kwon et al., 2011, Park and Kwon, 2011, Weiss et al., 2011, Weiss et al., 2011)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (2)

Stocks containing insertion(s) of this recombinant construct

Bloomington

57623

w^*; P{Gr33a-GAL4.4.7}1

57624

w^*; wg^Sp-1/CyO; P{Gr33a-GAL4.4.7}17

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Gr33a

(Israel et al., 2022)

Gr33a-GAL4

(Tadres et al., 2025, Luo et al., 2024, Miroschnikow et al., 2018, Choi et al., 2016, Ling et al., 2014, Kwon et al., 2011, Weiss et al., 2011)

Gr33a-Gal4

(Jacobs et al., 2024, Qian et al., 2018, Rist and Thum, 2017, Apostolopoulou et al., 2016, Kwon et al., 2014, Park and Kwon, 2011)

P{Gr33a-GAL4.4.7}

(FlyBase, 1992-)

Secondary FlyBase IDs

References (19)

Report Sections

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