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General Information
Symbol
Scer\GAL4Gr33a.4.7
Species
S. cerevisiae
Name
FlyBase ID
FBal0249481
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Gr33a-GAL4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 4.7kb Gr33a promoter fragment is fused upstream of sequence encoding a GAL4 driver.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Scer\GAL4Gr33a.4.7 drives expression in the following series of neurons. Larval gustatory receptor neuron of the epiphysis DP1, where it is coexpressed with Scer\GAL4Gr66a.1.8, Scer\GAL4Gr32a.3.7.PW, Scer\GAL4Gr93a.5, Scer\GAL4Gr22b.5.8, Scer\GAL4Gr39a.a.3.4, Scer\GAL4Gr39b.4.4, Scer\GAL4Gr9a.6.3, Scer\GAL4Gr22d.3.1, Scer\GAL4Gr22e.2.5, Scer\GAL4Gr28b.a.3, Scer\GAL4Gr39a.b.1, Scer\GAL4Gr58b.1, and Scer\GAL4Gr59d.4.1. Larval gustatory receptor neuron of the epiphysis DP2 where it is coexpressed with Scer\GAL4Gr66a.1.8, Scer\GAL4Gr32a.3.7.PW, Scer\GAL4Gr77a.4 and Scer\GAL4Gr93b.5.3. Larval gustatory receptor neuron of the hypophysis VP1 and VP2 where it is coexpressed with Scer\GAL4Gr66a.1.8 and Scer\GAL4Gr68a.PB. Larval gustatory receptor neuron of the hypopharyngeal organ PP1 and PP2 where it is coexpressed with Scer\GAL4Gr66a.1.8, Scer\GAL4Gr32a.3.7.PW, Scer\GAL4Gr22b.5.8, Scer\GAL4Gr39a.a.3.4, Scer\GAL4Gr39b.4.4, Scer\GAL4Gr39a.d.1.7, Scer\GAL4Gr93c.3.8 and Scer\GAL4Gr93d.2.4 Larval gustatory receptor neuron of the hypopharyngeal organ PP3 where it is coexpressed with Scer\GAL4Gr66a.1.8, Scer\GAL4Gr32a.3.7.PW, Scer\GAL4Gr28a.PT, Scer\GAL4Gr22b.5.8, Scer\GAL4Gr39a.a.3.4, Scer\GAL4Gr39b.4.4, Scer\GAL4Gr39a.d.1.7, Scer\GAL4Gr93c.3.8 and Scer\GAL4Gr93d.2.4 Larval gustatory receptor neuron of the hypopharyngeal organ PP4 where it is coexpressed with Scer\GAL4Gr66a.1.8, Scer\GAL4Gr28a.PT, Scer\GAL4Gr22b.5.8, Scer\GAL4Gr39a.a.3.4, Scer\GAL4Gr39b.4.4, Scer\GAL4Gr39a.d.1.7, Scer\GAL4Gr93c.3.8 and Scer\GAL4Gr93d.2.4.

Expression of UAS- mCD8::GFP via Scer\GAL4Gr33a.4.7 confirmed that it is expressed in 12 GRN pairs. These 12 pairs are suggested to represent the entire set of larval bitter receptor neurons. They are: (i) two pairs B1 and B2 in the dorsolateral group of sensilla of the TO, (ii) four pairs C1-C4 in the distal group of TO sensilla, (iii) two pairs D1 and D2 in the DPS (epiphysis), (iv) two pairs E1 and E2 in the VPS (hypophysis), and (v) two pairs F1 and F2 in the PPS (hypopharyngeal organ).

Scer\GAL4Gr33a.4.7 drives expression in adult abdominal multidendritic neurons with with dorsal processes extending along the left-right axis, as well as in multidendritic neurons with lateral processes extending anteroposteriorly; these major processes have several protruding dendrites. Expression is also observed in projections to the abdominal ganglion.

Scer\GAL4Gr33a.4.7 is expressed in the gustatory neurons of all of the bitter-sensitive short labellar taste bristles, and the gustatory neurons of all of the intermediate labellar taste bristles.

Scer\GAL4Gr33a.4.7 is expressed in all of the short and intermediate bitter-sensitive sensilla of the labellum.

Scer\GAL4Gr33a.4.7 drives expression in 6 gustatory receptor neurons of the larval terminal organ, two of these having its cell body in the dorsal organ ganglion (TO-dorsolateral group). All 6 neurons co-express Scer\GAL4Gr66a.1.8. One of the neurons of the TO-dorsolateral group also expresses Scer\GAL4Gr10a.3.7 and Scer\GAL4Gr32a.3.7.PW. The other terminal organ neurons express different combinations of drivers. The first neuron expresses Scer\GAL4Gr32a.3.7.PW, Scer\GAL4Gr28b.e.4.245, Scer\GAL4Gr39a.a.3.4, Scer\GAL4Gr39a.b.1, Scer\GAL4Gr59d.4.1, Scer\GAL4Gr9a.6.3, Scer\GAL4Gr22e.2.5, Scer\GAL4Gr22a.4.2, Scer\GAL4Gr28b.a.3, Scer\GAL4Gr36b.0.9, Scer\GAL4Gr36c.2.5, Scer\GAL4Gr58b.1, Scer\GAL4Gr59a.1.5, Scer\GAL4Gr59c.5.5 and Scer\GAL4Gr93b.5.3. The second neuron co-expresses Scer\GAL4Gr39a.a.3.4, Scer\GAL4Gr57a.6, Scer\GAL4Gr59d.4.1, Scer\GAL4Gr47b.2 and Scer\GAL4Gr94a.3.7. The third neuron co-expresses Scer\GAL4Gr57a.6 and Scer\GAL4Gr97a.1. The fourth neuron expresses Scer\GAL4Gr39a.b.1 and Scer\GAL4Gr59d.4.1. Expression of Scer\GAL4Gr33a.4.7 is also found in two neurons of the dorsal pharyngeal sense organ (DPS or epiphysis), two neurons of the posterior pharyngeal sense organ (PPS or hypopharyngeal sense organ) and in two neurons of the ventral pharyngeal sense organ (VPS or hypophysis).

 
Marker for
Reporter construct used in assay
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Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
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Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
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Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Scer\GAL4Gr33a.4.7
Name Synonyms
Secondary FlyBase IDs
    References (11)