FB2026_03 , released September 17, 2026
Recombinant Construct: PBac{UAS-Ub-FT-nSyb}
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General Information
Symbol
PBac{UAS-Ub-FT-nSyb}
FlyBase ID
FBtp0097409
Feature type
transgenic_transposable_element
Component Allele(s)
nSybUAS.Ubi.slowFT
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele nSybUAS.Ubi.slowFT
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
nSyb
Tagged with
slowFT
Description

UAS regulatory sequences drive expression of a fusion protein consisting of amino acid residues 1-74 of RpL40, a Gln-Gln-Ser-Arg spacer, slowFT (a fluorescent timer protein that changes from blue to red emission over time, allowing protein turnover to be analysed) and the nSyb coding region. The RpL40 portion encodes ubiquitin (minus the C-terminal Gly residues, to prevent its removal by deubiquitinases), which targets the fusion protein for degradation.

(Fernandes et al., 2014)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{UAS-Ub-FT-nSyb}
(FlyBase, 1992-)
UAS-Ub-FT-nSyb
(Fernandes et al., 2014)
Secondary FlyBase IDs
    References (3)