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General Information
Symbol
P{UAS-CaMPARI}
FlyBase ID
FBtp0098285
Feature type
transgenic_transposon
Component Allele(s)
Also Known As
UAS-CaMPARI
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Component allele Lhem\EosFPCaMPARI.UAS
Product class / Tool use(s)
Description

UAS regulatory sequences drive expression of the photoconvertible CaMPARI fluorescent Ca[2+] indicator.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

Scer\UAS regulatory sequences drive expression of the "CaMPARI" fluorescent Ca[2+] indicator, which undergoes efficient and irreversible green-to-red conversion only in the presence of both elevated intracellular Ca[2+] levels and experimenter-supplied violet light. "CaMPARI" (calcium-modulated photoactivatable ratiometric integrator) consists of a nuclear export signal (T:Uuuu\nes-Unk), the Ca[2+]-binding protein T:Rat\Cam, a circularly permuted variant of mEos2 and the T:Ggal\MLCK M13 peptide. The circularly permuted mEos2 sequence carries the following additional mutations relative to mEos2: V2 insert, F34Y, S39T, A69V, L93M, I102Y, E140M, T143W, M146I, A160I, Y189V and H213Y.

Stocks (2)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (10)