FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{UDMEL-Myd88T-dahA}
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General Information
Symbol
M{UDMEL-Myd88T-dahA}
FlyBase ID
FBtp0111364
Feature type
transgenic_transposable_element
Component Allele(s)
dahbnk.A
Myd88RNAi.bcd.T
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Myd88RNAi.bcd.T
Transgenic product class
RNAi_reagent
(Akbari et al., 2013)
Component
Use(s)
Regulatory region(s)
bcd
Encoded product / tool
Myd88
Description

A maternal germline specific promoter (bcd) drives expression of a 4 x 2 modified forms of mir-6-1 that have been modified to target different sites in the Myd88 5' untranslated region. This allele is used as a 'toxin' in the maternal-effect-lethal underdominance (UD[MEL]) system.

(Akbari et al., 2013)
Component allele dahbnk.A
Transgenic product class
wild_type
(Akbari et al., 2013)
Component
Use(s)
Regulatory region(s)
bnk
Encoded product / tool
dah
Description

A transient early-zygotic promoter (bnk) drives expression of dah lacking the 5' UTR, which prevents it from being silenced by one of the sequences targeted by dahmiRNA.bcd.T. To prevent it being silenced by the second miRNA in dahmiRNA.bcd.T, which targets an exon, the nucleotide sequence has been recoded such that it codes for the same amino acid sequence but is not targeted by the miRNA. This allele is used as an 'antidote' in the maternal-effect-lethal underdominance (UD[MEL]) system.

(Akbari et al., 2013)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

This construct is used as part of the maternal-effect-lethal underdominance (UD[MEL]) system. The U[DMEL] system utilizes two constructs, each consisting of a maternally expressed toxin and a zygotically expressed antidote. Each antidote is linked with a toxin whose activity it does not rescue (toxin A linked with antidote B and vice versa). As a result, the survival of embryos from mothers carrying one (UD[MEL-1]) or both (UD[MEL-1] and UD[MEL-2]) kinds of UD[MEL] chromosomes requires that they inherit the other (UD[MEL-2]) or both kinds of UD[MEL] chromosomes, respectively, in order to achieve zygotic rescue.

(Akbari et al., 2013)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
M{UDMEL-Myd88T-dahA}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)