FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-3xFLAG.dCas9.VPR}
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General Information
Symbol
P{UAS-3xFLAG.dCas9.VPR}
FlyBase ID
FBtp0114515
Feature type
transgenic_transposable_element
Component Allele(s)
Spyo\Cas9dCas9::VPR.UAS.Tag:FLAG
Also Known As
UAS-dCas9-VPR, dCas9-VPR, UAS:dCas9-VPR, UAS-dCas9.VPR, UAS-dCas9, UAS-dCas9VPR
Size
Expression Data
Associated insertion(s)
3 available
Molecular map
Description and Uses
Construct components
Component allele Spyo\Cas9dCas9::VPR.UAS.Tag:FLAG
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
dCas9::VPR
Tagged with
Tag:FLAG
Tag:NLS(Unk)
Description

10x UAS regulates expression of the artificial transcriptional activator dCas9::VPR, which is composed of a nuclease-dead form of Spyo\Cas9 fused to a highly active transcription activation sequence. The driver is tagged at the N-terminus with 3x Tag:FLAG and nuclear localization signals are present either side of the mutated Spyo\Cas9 sequence. This driver is designed to be used to overexpress a target gene of interest from its endogenous genomic locus, by combining it with sgRNAs that target sequences upstream of the transcription start site of the gene of interest.

(Lin et al., 2015)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (32)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (17)
References (29)