FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{loxP.BB(hs-neo)}
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General Information
Symbol
M{loxP.BB(hs-neo)}
FlyBase ID
FBtp0116194
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

Target construct for recombination-mediated cassette exchange (RMCE) using the Bxb1:int integrase. Contains a Tn\neoRHsp70Bb.PS marker flanked by a pair of inverted 48bp minimal attP(Bxb1) sites. The entire attP(Bxb1)-flanked cassette can be replaced with DNA from a compatible donor plasmid (where the sequence to be inserted is flanked by inverted attB(Bxb1) sites) through RMCE driven by the Bxb1:int integrase. A single loxP site is also present, immediately preceding the RMCE cassette.

(Voutev and Mann, 2017)
Construct components
Component
Use(s)
Element type
Also carries
attP(Bxb1)
loxP
Marker(s)
Tn\neoRHsp70Bb.PS
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
M{3xP3-RFP.attP}
(Voutev and Mann, 2017)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
BBhs-neo
(Voutev and Mann, 2017)
M{loxP.BB(hs-neo)}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)