FB2026_02 , released June 18, 2026
Recombinant Construct: P{UAS(-loxP)QF}
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General Information
Symbol
P{UAS(-loxP)QF}
FlyBase ID
FBtp0149856
Feature type
transgenic_transposable_element
Component Allele(s)
Ncra\QFUAS.-loxP
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

The P{UAS(loxP.lexA)QF} mosaic expression system is designed to generate mosaic tissues composed of clones which express either the lexA::VP16 driver or the QF driver. The P{UAS(loxP.lexA)QF} construct has the following structure: UAS regulatory sequences - loxP site - lexA::VP16 driver sequence (with polyadenylation signal) - loxP site - QF driver sequence (with polyadenylation signal). In the absence of P1cre recombinase, only the lexA::VP16 driver is expressed (represented in FlyBase by the Ecol\lexAVP16.UAS.loxP allele). If excision of the loxP-flanked sequence occurs (generating the P{UAS(-loxP)QF} construct), the lexA::VP16 driver sequence is removed, allowing expression of the QF driver instead (represented in FlyBase by the Ncra\QFUAS.-loxP allele).

(Banzai et al., 2022)
Construct components
Component allele Ncra\QFUAS.-loxP
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
QF
Also carries
loxP
Description

The loxP cassette present in P{UAS(loxP.lexA)QF} has been excised, removing the lexA::VP16 driver sequence and allowing expression of the QF driver under the control of UAS regulatory sequences.

(Banzai et al., 2022)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
P{UAS(loxP.lexA)QF}
(Banzai et al., 2022)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{UAS(-loxP)QF}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)