Open Close
General Information
Symbol
QF
FlyBase ID
FBto0000167
Name
Description
Description

Neurospora crassa QA-1F (UniProtKB:P11638) is a transcriptional activator that contains both a sequence-specific DNA-binding domain and a transcriptional activation domain (reviewed in PMID:1825499). QA-1F binds specifically to the QUAS regulatory sequence. This property can be utilized to form a binary expression system to control the spatial and temporal expression of a gene of interest: a transgene or modified endogenous locus in which the target gene of interest is downstream of QUAS sequences is combined with a transgene or modified endogenous locus encoding a compatible 'QF driver'. The QF entry in FlyBase represents a driver corresponding to either the naturally occurring QA-1F protein, or where the precise variant is unknown/unspecified, but both DNA-binding domain and transcriptional activation domain are derived from QA-1F. The exact sequence of the driver may thus differ depending on the particular transgene or modified endogenous locus being used. The expression pattern of a gene of interest can be further refined by adding the QS suppressor, which binds to QF such that although it can still bind the QUAS sequence it can no longer activate transcription. The suppression of QF by QS can itself be suppressed by feeding flies with quinic acid, providing a further level of regulation (FBrf0210697, reviewed in FBrf0214230, FBrf0233764).

External Crossreferences and Linkouts
Related experimental tools
Transgenic Constructs
Encodes tool (28)
Transgenic construct(s)
Component allele
Reg. region
Encoded product / tool
Tagged with
Also carries
Stocks
Tagged with the tool (1)
Transgenic construct(s)
Component allele
Reg. region
Encoded product / tool
Tagged with
Also carries
Stocks
Insertions ( 33 )
Insertion
Encoded product / tool
Tagged with
Also carries
Stocks
Associated Alleles
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
QF
Name Synonyms
Secondary FlyBase IDs
    References (24)