The Scer\FRT cassette has been excised from P{Trh(FRT.stop)QF}, allowing the Ncra\QF coding region to be expressed under the control of Trh regulatory sequences. A P1\loxP cassette containing a w[+] marker is present downstream of the Ncra\QF coding region.
The Scer\FRT cassette has been excised from P{Trh(FRT.stop)QF}, allowing the Ncra\QF coding region to be expressed under the control of Trh regulatory sequences. A P1\loxP cassette containing a w[+] marker is present downstream of the Ncra\QF coding region.