Trh regulatory sequences are separated from the QF coding region (followed by SV40 transcription termination sequence) by an FRT cassette containing an SV40 transcription termination sequence and an α tubulin transcription termination sequence.
Trh regulatory sequences are separated from the Ncra\QF coding region (followed by SV40 transcription termination sequence) by an Scer\FRT cassette containing an SV40 transcription termination sequence and an α tubulin transcription termination sequence. A P1\loxP cassette containing a w[+] marker is present downstream of the Ncra\QF coding region.
Trh regulatory sequences are separated from the Ncra\QF coding region (followed by SV40 transcription termination sequence) by an Scer\FRT cassette containing an SV40 transcription termination sequence and an α tubulin transcription termination sequence. A P1\loxP cassette containing a w[+] marker is present downstream of the Ncra\QF coding region.