FB2026_02 , released June 18, 2026
Experimental tool: QF(DBD)::Zip-
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General Information
Symbol
QF(DBD)::Zip-
FlyBase ID
FBto0000525
Name
Description
Description

The QF(DBD)::Zip- entry in FlyBase represents a hemidriver that contains the DNA-binding domain from the Neurospora crassa QA-1F gene (UniProtKB:P11638) fused to the Zip- heterodimerizing leucine zipper (the RR[[12]]EE[[345]]L zipper from PMID:11344333). It forms one half of a 'split driver' system: when combined in vivo with a hemidriver containing a transcriptional activation domain fused to the Zip+ heterodimerizing leucine zipper (the EE[[12]]RR[[345]]L zipper from PMID:11344333), the interaction between the Zip+/Zip- heterodimerizing pair can reconstitute a functional transcriptional activator that can drive transcription of a downstream gene of interest. Since transcription is only activated at the intersection of the expression patterns of the two hemidrivers, a split driver system can be used to drive expression in a restricted pattern. The QF(DBD)::Zip- hemidriver is compatible with any activation domain hemidriver containing the Zip+ domain. The reconstituted transcriptional activator binds specifically to the QUAS regulatory sequence due to the presence of the QA-1F DNA-binding domain (FBrf0242216).

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Transgenic Constructs
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Transgenic construct(s)
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Insertions ( 1 )
Insertion
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Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
QF(DBD)::Zip-
Name Synonyms
Secondary FlyBase IDs
    References (3)