The GAL4(AD)::Zip+ entry in FlyBase represents a hemidriver that contains a transcriptional activation domain from the Saccharomyces cerevisiae GAL4 gene ( SGDID:S000006169 ) fused to the Zip+ heterodimerizing leucine zipper (the EE[[12]]RR[[345]]L zipper from PMID:11344333). The exact sequence of the hemidriver may differ depending on the particular transgene or modified endogenous locus being used. For example, while a nuclear localization signal must be present in the hemidriver, its origin may vary. GAL4(AD)::Zip+ forms one half of a 'split driver' system: when combined in vivo with a hemidriver containing a DNA-binding domain fused to the Zip- heterodimerizing leucine zipper (the RR[[12]]EE[[345]]L zipper from PMID:11344333), the interaction between the Zip+/Zip- heterodimerizing pair can reconstitute a functional transcriptional activator that can drive transcription of a downstream gene of interest. Since transcription is only activated at the intersection of the expression patterns of the two hemidrivers, a split driver system can be used to drive expression in a restricted pattern. The GAL4(AD)::Zip+ hemidriver is compatible with any DNA-binding domain hemidriver containing the Zip- domain. The DNA-binding specificity of the reconstituted transcriptional activator will depend upon the DNA-binding domain present in the DNA-binding domain Zip- hemidriver. In addition, since the GAL4(AD)::Zip+ hemidriver includes the GAL4 sequence targeted by the GAL80 suppressor, the reconstituted transcriptional activator can be suppressed by GAL80 (FBrf0193617).