FB2026_02 , released June 18, 2026
Recombinant Construct: P{CFD4FLPOUT-Lk6-MAPk-Ak2}
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General Information
Symbol
P{CFD4FLPOUT-Lk6-MAPk-Ak2}
FlyBase ID
FBtp0166041
Feature type
transgenic_transposable_element
Component Allele(s)
Lk6U6.Lk6+MAPk-Ak2.CFD4FLPOUT
MAPk-Ak2U6.Lk6+MAPk-Ak2.CFD4FLPOUT
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Description

The P{CFD4FLPOUT-Lk6-MAPk-Ak2} construct contains two sgRNAs, with each flanked by a distinct fluorescent reporter. The transgene also contains two pairs of mutually incompatible FRT sites (FRT and FRT3) arranged such that a single FLP-mediated recombination event excises one of the two sgRNAs (plus one of the reporters) in a stochastic manner, with no further recombination possible. The construct has the following structure: FRT site - Avic\GFP3xP3.cUa marker - sgRNA1 (Lk6U6.Lk6+MAPk-Ak2.CFD4FLPOUT) - FRT3 site - FRT site - sgRNA2 (MAPk-Ak2U6.Lk6+MAPk-Ak2.CFD4FLPOUT) - Disc\RFPmCh.3xP3.cUa marker - FRT3 site.

(Transgenic RNAi Project, 2024.1.30)
Construct components
Component
Use(s)
Also carries
FRT
FRT3
Marker(s)
Avic\GFP3xP3.cUa
Disc\RFPmCh.3xP3.cUa
Component allele Lk6U6.Lk6+MAPk-Ak2.CFD4FLPOUT
Transgenic product class
sgRNA
(Transgenic RNAi Project, 2024.1.30)
Component
Use(s)
Encoded product / tool
Lk6
Description

U6 promoter sequences drive expression of a Lk6 sgRNA (CCTTCATTCCTCAACCTTTCAGG).

(Transgenic RNAi Project, 2024.1.30)
Component allele MAPk-Ak2U6.Lk6+MAPk-Ak2.CFD4FLPOUT
Transgenic product class
sgRNA
(Transgenic RNAi Project, 2024.1.30)
Component
Use(s)
Encoded product / tool
MAPk-Ak2
Description

U6 promoter sequences drive expression of a MAPk-Ak2 sgRNA (ATCCGCTGACCCGCCAATGCAGG).

(Transgenic RNAi Project, 2024.1.30)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
602804
y1 v1; P{CFD4FLPOUT-Lk6-MAPk-Ak2}attP40/CyO
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{CFD4FLPOUT-Lk6-MAPk-Ak2}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)