FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
CV Term Report
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General Information
Term hydrolase activity ID (Ontology) GO:0016787 (Gene Ontology)
Definition Catalysis of the hydrolysis of various bonds, e.g. C-O, C-N, C-C, phosphoric anhydride bonds, etc.[ ISBN:0198506732 ]
Comment
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Annotations
Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
Data Class Field Records
Genes (FBgn)  GO_MOLECULAR_FUNCTION     103
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 Full annotation statements 
Relevant FlyBase reports
 Gene Groups Genes
 hydrolase activity (all annotations which use CV term, excluding "NOT" statements)       1    103
     enables | hydrolase activity       --     103
 NOT | enables | hydrolase activity       --       3
Spanning Tree (Parents/Children)
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  molecular_function
   |__catalytic activity
       |__hydrolase activity  1858 rec.
           |__cytosine C-5 DNA demethylase activity
           |__hydrolase activity, acting on acid anhydrides 458 rec.
           |   |__hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides(+) 456 rec.
           |   |__hydrolase activity, acting on acid anhydrides, in sulfonyl-containing anhydrides(+)
           |__hydrolase activity, acting on carbon-carbon bonds 5 rec.
           |   |__6-hydroxycyclohex-1-ene-1-carboxyl-CoA hydratase activity
           |   |__hydrolase activity, acting on carbon-carbon bonds, in ketonic substances(+) 4 rec.
           |__hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds 107 rec.
           |   |__(S)-norcoclaurine synthase activity
           |   |__deacetylipecoside synthase activity
           |   |__deacetylisoipecoside synthase activity
           |   |__deaminase activity(+) 21 rec.
           |   |__hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides(+) 6 rec.
           |   |__hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines(+) 23 rec.
           |   |__hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides(+) 71 rec.
           |   |__hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines(+) 3 rec.
           |   |__hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in nitriles(+)
           |   |__hydroxydechloroatrazine ethylaminohydrolase activity
           |   |__IAA-amino acid conjugate hydrolase activity(+)
           |   |__jasmonic acid hydrolase activity
           |   |__jasmonyl-Ile conjugate hydrolase activity
           |   |__N-acetyl-anhydromuramoyl-L-alanine amidase activity
           |   |__N-isopropylammelide isopropylaminohydrolase activity
           |   |__riboflavinase activity
           |   |__strictosidine synthase activity
           |   |__thiaminase activity
           |__hydrolase activity, acting on carbon-phosphorus bonds
           |   |__phosphonoacetaldehyde hydrolase activity
           |   |__phosphonoacetate hydrolase activity
           |   |__phosphonopyruvate hydrolase activity
           |__hydrolase activity, acting on carbon-sulfur bonds 2 rec.
           |   |__2'-hydroxybiphenyl-2-sulfinate desulfinase activity
           |   |__adenosylhomocysteinase activity 1 rec.
           |   |__UDPsulfoquinovose synthase activity
           |__hydrolase activity, acting on ester bonds 546 rec.
           |   |__carboxylic ester hydrolase activity(+) 154 rec.
           |   |__deoxyribonuclease (pyrimidine dimer) activity
           |   |__DNA endonuclease activity, producing 3'-phosphomonoesters(+) 4 rec.
           |   |__DNA endonuclease activity, producing 5'-phosphomonoesters(+) 10 rec.
           |   |__double-stranded DNA endonuclease activity(+) 13 rec.
           |   |__double-stranded RNA-specific ribonuclease activity(+) 5 rec.
           |   |__enterochelin esterase activity
           |   |__excinuclease ABC activity
           |   |__exonuclease activity(+) 56 rec.
           |   |__flap endonuclease activity(+) 6 rec.
           |   |__lipase activity(+) 105 rec.
           |   |__N-acetyltaurine hydrolase activity 1 rec.
           |   |__phosphoric ester hydrolase activity(+) 249 rec.
           |   |__restriction endodeoxyribonuclease activity(+)
           |   |__retroviral 3' processing activity
           |   |__ribonuclease MRP activity 2 rec.
           |   |__RNA endonuclease activity producing 3'-phosphomonoesters, hydrolytic mechanism(+)
           |   |__RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism(+) 23 rec.
           |   |__single-stranded DNA endonuclease activity(+) 17 rec.
           |   |__sulfuric ester hydrolase activity(+) 12 rec.
           |   |__thiolester hydrolase activity(+) 19 rec.
           |   |__versiconal hemiacetal acetate esterase activity
           |__hydrolase activity, acting on ether bonds 9 rec.
           |   |__ether hydrolase activity(+) 8 rec.
           |   |__trialkylsulfonium hydrolase activity(+)
           |__hydrolase activity, acting on glycosyl bonds 130 rec.
           |   |__hydrolase activity, hydrolyzing N-glycosyl compounds(+) 21 rec.
           |   |__hydrolase activity, hydrolyzing O-glycosyl compounds(+) 109 rec.
           |__hydrolase activity, acting on halide bonds
           |   |__diisopropyl-fluorophosphatase activity
           |   |__hydrolase activity, acting on halide bonds, in C-halide compounds(+)
           |__hydrolase activity, acting on phosphorus-nitrogen bonds 7 rec.
           |   |__phosphoamidase activity
           |   |__protein arginine phosphatase activity 2 rec.
           |   |__protein histidine phosphatase activity 5 rec.
           |__hydrolase activity, acting on sulfur-nitrogen bonds 2 rec.
           |   |__cyclamate sulfohydrolase activity
           |   |__N-sulfoglucosamine sulfohydrolase activity 1 rec.
           |__hydrolase activity, acting on sulfur-sulfur bonds
           |   |__trithionate hydrolase activity
           |__palmitoyl hydrolase activity 6 rec.
           |   |__palmitoyl-(protein) hydrolase activity 5 rec.
           |__peptidase activity 598 rec.
           |   |__aspartic-type peptidase activity(+) 30 rec.
           |   |__ATP-dependent peptidase activity 7 rec.
           |   |__cysteine-type peptidase activity(+) 102 rec.
           |   |__endopeptidase activity(+) 382 rec.
           |   |__exopeptidase activity(+) 108 rec.
           |   |__glutamic-type peptidase activity(+)
           |   |__metallopeptidase activity(+) 164 rec.
           |   |__omega peptidase activity(+) 12 rec.
           |   |__serine-type peptidase activity(+) 264 rec.
           |   |__threonine-type peptidase activity(+) 24 rec.
           |   |__ubiquitin-like protein peptidase activity(+) 77 rec.
           |__protein-phosphatidylethanolamide deconjugating activity 3 rec.
           |__serine hydrolase activity 326 rec.
           |   |__serine-type peptidase activity(+) 264 rec.
           |__steryl deacetylase activity
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EC:3.-.-.-
Reactome:R-HSA-1236938 "Partial proteolysis of antigen in phagolysosomes"
Reactome:R-HSA-2029475 "Production of AA by iPLA2 upon FCGR activation"
Reactome:R-HSA-5694583 "ABHD4 hydrolyses NAPE"
Reactome:R-HSA-5695964 "ABHD14B hydrolyses PNPB"
Reactome:R-HSA-6786190 "CMBL hydrolyses OM to OLMS"
Reactome:R-HSA-6788295 "HDHD1:Mg2+ dephosphorylates PURIDP"
Reactome:R-HSA-8938314 "ENPPs hydrolyse CoA-SH to PPANT, PAP"
Reactome:R-HSA-8952137 "Phospholipid phosphatase 6 hydrolyses Presqualene diphosphate to presqualene monophosphate"
Reactome:R-HSA-9942295 "QNG1 hydrolyzes queuosine 5'-monophosphate yielding queuine and ribose 5'-monophosphate"