FB2026_02 , released June 18, 2026

CV Term Report

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General Information

Term

transcriptional complementation assay

ID (Ontology)

MI:0232 (Molecular Interactions)

Definition

Protein complementation assay performed by dissecting a transcription factor activity (DNA binding domain and transcription activation domain) its restoration through the two hybrid proteins interaction that lead to a reporter gene expression.[ PubMed:14755292 ]

Also Known As

"transcription compl"

Comment

Links to External Ontologies

Annotations

Records annotated with this term OR any of its CHILD TERMS

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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records

Full annotation statements	Relevant FlyBase reports
Full annotation statements	Interaction Groups
transcriptional complementation assay (all annotations which use CV term, excluding "NOT" statements)

Spanning Tree (Parents/Children)

  experimental interaction detection
   |__protein complementation assay
       |__transcriptional complementation assay  5400 rec.
           |__lambda repressor two hybrid
           |__lex-a dimerization assay
           |__membrane yeast two hybrid 4 rec.
           |   |__ire1 reconstruction
           |   |__ubiquitin reconstruction 4 rec.
           |__one hybrid 9 rec.
           |__reverse two hybrid
           |__three hybrid 54 rec.
           |   |__protein three hybrid 17 rec.
           |   |__rna three hybrid 21 rec.
           |__two hybrid 5363 rec.
               |__gal4 vp16 complementation
               |__lexa b52 complementation
               |__lexa vp16 complementation
               |__protein three hybrid 17 rec.
               |__two hybrid array 1984 rec.
               |__two hybrid pooling approach(+)
               |__validated two hybrid

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Relationships
Is a	protein complementation assay
Part of
Synonyms & Secondary IDs
Synonyms
	"transcription compl" EXACT PSI-MI-short
Secondary IDs

External Crossreferences & Linkouts