FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
CV Term Report
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General Information
Term transcriptional complementation assay ID (Ontology) MI:0232 (Molecular Interactions)
Definition Protein complementation assay performed by dissecting a transcription factor activity (DNA binding domain and transcription activation domain) its restoration through the two hybrid proteins interaction that lead to a reporter gene expression.[ PubMed:14755292 ]
Also Known As "transcription compl"
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 transcriptional complementation assay (all annotations which use CV term, excluding "NOT" statements)       2
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  experimental interaction detection
   |__protein complementation assay
       |__transcriptional complementation assay  5400 rec.
           |__lambda repressor two hybrid
           |__lex-a dimerization assay
           |__membrane yeast two hybrid 4 rec.
           |   |__ire1 reconstruction
           |   |__ubiquitin reconstruction 4 rec.
           |__one hybrid 9 rec.
           |__reverse two hybrid
           |__three hybrid 54 rec.
           |   |__protein three hybrid 17 rec.
           |   |__rna three hybrid 21 rec.
           |__two hybrid 5363 rec.
               |__gal4 vp16 complementation
               |__lexa b52 complementation
               |__lexa vp16 complementation
               |__protein three hybrid 17 rec.
               |__two hybrid array 1984 rec.
               |__two hybrid pooling approach(+)
               |__validated two hybrid
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Is a protein complementation assay
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Synonyms
  • "transcription compl" EXACT PSI-MI-short
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