FlyBase Allele Report: Dmel\ph-p[GD4480]

General Information

Symbol

Dmel\ph-p^GD4480

Species

D. melanogaster

Name

FlyBase ID

FBal0210332

Feature type

allele

Associated gene

Dmel\ph-p

Associated Insertion(s)

Carried in Construct

P{GD4480}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4480}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4480

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Sievers et al., 2014)

CEA with aPKC^{UAS.Tag:CAAX(Unk)}

(Bunker et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by dlg1², dlg1^A40.2

(Bunker et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

neoplasia | heat sensitive, with Scer\GAL4^en-e16E

(Sievers et al., 2014)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

wing disc posterior compartment | heat sensitive, with Scer\GAL4^en-e16E

(Sievers et al., 2014)

Detailed Description

Statement

Reference

The expression of ph-p^GD4480 under the control of Scer\GAL4^en-e16E does not lead to significant size defects in the posterior compartment of third instar larval wing discs, as compared to controls.

(Bunker et al., 2015)

Expression of ph-p^GD4480 within the posterior compartment of the wing imaginal disc under the control of Scer\GAL4^en-e16E leads to tumor formation. The probability of tumor development is approximately 0.15. The tumors exhibit extensive morphological anomalies and are characterised by fast growth, hight cell density, and loss of tissue polarity.

(Sievers et al., 2014)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4480

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dcr-2^UAS.cDa, Scer\GAL4^en-e16E, ph-p^GD4480 has neoplasia phenotype, suppressible by Dcr-2^UAS.cDa/Su(H)^NIG.3497R, Scer\GAL4^en-e16E

(Saj et al., 2010)

Enhancer of

Statement

Reference

Scer\GAL4^en-e16E/ph-p^GD4480 is an enhancer of neoplasia | third instar larval stage phenotype of dlg1²

(Bunker et al., 2015)

Other

Statement

Reference

Scer\GAL4^Bx-MS1096, aPKC^{UAS.Tag:CAAX(Unk)}, ph-p^GD4480 has neoplasia | third instar larval stage phenotype

(Bunker et al., 2015)

Dcr-2^UAS.cDa, Scer\GAL4^en-e16E, ph-p^GD4480 has neoplasia | third instar larval stage phenotype

(Saj et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dcr-2^UAS.cDa, Scer\GAL4^en-e16E, ph-p^GD4480 has wing disc | third instar larval stage phenotype, suppressible by Dcr-2^UAS.cDa/Su(H)^NIG.3497R, Scer\GAL4^en-e16E

(Saj et al., 2010)

Enhancer of

Statement

Reference

Scer\GAL4^en-e16E/ph-p^GD4480 is an enhancer of wing disc | third instar larval stage phenotype of dlg1²

(Bunker et al., 2015)

Other

Statement

Reference

Scer\GAL4^Bx-MS1096, aPKC^{UAS.Tag:CAAX(Unk)}, ph-p^GD4480 has wing disc | third instar larval stage phenotype

(Bunker et al., 2015)

Dcr-2^UAS.cDa, Scer\GAL4^en-e16E, ph-p^GD4480 has wing disc | third instar larval stage phenotype

(Saj et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of ph-p^GD4480 under the control of Scer\GAL4^en-e16E enhances the tumor phenotype of dlg1²/dlg1^A40.2 transheterozygous third instar larval wing discs, as it leads to a significantly increased cell number in the discs' posterior compartment.

The co-expression of ph-p^GD4480 and aPKC^{CAAXWT.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 leads to a severe overgrowth of third instar larval wing discs, as compared to controls.

(Bunker et al., 2015)

Wing discs co-expressing ph-p^GD4480 and Dcr-2^Scer\UAS.cDa driven by Scer\GAL4^en-e16E display large tumors.

Co-expression of Su(H)^NIG.3497R results in an almost complete suppression of the overgrowth phenotype caused by the co-expression of ph-p^GD4480 with Dcr-2^Scer\UAS.cDa driven by Scer\GAL4^en-e16E.

(Saj et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v10679

w¹¹¹⁸; P{GD4480}v10679

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4480

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ph-p^GD4480

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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