FB2025_01 , released February 20, 2025
Allele: Dmel\Srrm1KK108396
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General Information
Symbol
Dmel\Srrm1KK108396
Species
D. melanogaster
Name
FlyBase ID
FBal0235545
Feature type
allele
Associated gene
Dmel\Srrm1
Associated Insertion(s)
Carried in Construct
P{KK108396}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
in vitro construct
(Keleman et al., 2009.8.5)
Progenitor genotype
Carried in construct
P{KK108396}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK108396
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of SRm160KK108396 alone or in combination with SRm160GL00538 (using UAS-dicer2 to enhance the RNAi efficiency) under the control of either the Scer\GAL4P2.4.Pdf or the Scer\GAL4tim.PE driver results in strong reduction in the proportion of rhythmic flies and decrease in the rhythmic power of the behavioral oscillations under free-running (constant darkness) conditions in the adult locomotor activity assay. Under driven light-dark conditions, SRm160 knockdown does not lead to any significant irregularities in the locomotor activity, the morning and evening anticipatory behavior is slightly diminished but not statistically significant. Scer\GAL4P2.4.Pdf-controlled expression of SRm160KK108396 does not lead to any structural defects to the LNv neuronal projections but it abrogates the oscillations of Pdf protein levels in the projections of s-LNv neurons and also dampens the oscillation of the per protein in these neurons.

      Expression of SRm160KK108396 limited to adulthood by employing the Scer\GAL4Pdf.Switch1a driver and feeding the flies RU486 to activate the expression also results in significant decrease in the percentage of rhythmic flies.

      (Beckwith et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The loss of rhythmicity in locomotor activity under free-running (constant darkness) conditions characteristic for flies expressing SRm160KK108396 (and UAS-dicer2 to enhance the RNAi efficiency) under the control of Scer\GAL4P2.4.Pdf is significantly improved by co-expression of perScer\UAS.cYa but not by co-expression of ClkScer\UAS.cZa or by addition of a single copy of either perN.T:Ivir\HA1 or ClkT:SV5\V5.

      (Beckwith et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      SRm160KK108396
      Srrm1KK108396
      Name Synonyms
      Secondary FlyBase IDs
        References (5)