FlyBase Allele Report: Dmel\Nmdar1[RNAi.UAS.cWa]

General Information

Symbol

Dmel\Nmdar1^RNAi.UAS.cWa

Species

D. melanogaster

Name

FlyBase ID

FBal0268132

Feature type

allele

Associated gene

Dmel\Nmdar1

Associated Insertion(s)

Carried in Construct

P{UAS-Nmdar1.RNAi.W}

Also Known As

UAS-dsNR1

Key Links

Transgenic product class

RNAi_reagent

(Wu et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Wu et al., 2007)

Mutagen

in vitro construct

(Wu et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Nmdar1.RNAi.W}

Cytology

Description

UAS regulatory sequences drive expression of a Nmdar1 dsRNA.

(Wu et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Nmdar1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory, with Scer\GAL4^ey-OK107

(Wu et al., 2007)

abnormal memory, with Scer\GAL4^Feb170

(Wu et al., 2007)

abnormal memory | heat sensitive, with Scer\GAL4^hs.PU

(Wu et al., 2007)

Phenotype Manifest In

Detailed Description

Statement

Reference

Long term memory appears to be abolished, but anesthesia-resistant memory is normal in flies expressing Nmdar1^{dsRNA.Scer\UAS.cWa} under the control of Scer\GAL4^hs.PU after heat shock.

1 day memory is impaired after spaced training, but not after massed training in an olfactory associative conditioning assay in flies expressing Nmdar1^{dsRNA.Scer\UAS.cWa} under the control of Scer\GAL4^Feb170. 3 hour memory after one session of training is normal in these flies.

1 day memory after spaced or massed training in an olfactory associative conditioning assay is not affected in flies expressing Nmdar1^{dsRNA.Scer\UAS.cWa} under the control of Scer\GAL4^ey-OK107. 3 hour anesthesia-sensitive memory is impaired in these flies compared to controls.

(Wu et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Nmdar1^RNAi.UAS.cWa, Scer\GAL4^GH146 is an enhancer of abnormal smell perception | heat sensitive phenotype of Scer\GAL4^GH146, TrpA1^UAS.(B).cKa

(Sudhakaran et al., 2012)

Nmdar1^RNAi.UAS.cWa, Scer\GAL4^VPN is an enhancer of abnormal smell perception | heat sensitive phenotype of Scer\GAL4^VPN, TrpA1^UAS.(B).cKa

(Sudhakaran et al., 2012)

Other

Statement

Reference

Nmdar1^RNAi.UAS.cWa, Nmdar2^RNAi.UAS.cWa, Scer\GAL4^G0431 has abnormal memory phenotype

(Chen et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

1 day memory is impaired after spaced training, but not after massed training in an olfactory associative conditioning assay in flies co-expressing Nmdar1^{dsRNA.Scer\UAS.cWa} and Nmdar2^{dsRNA.Scer\UAS.cWa} under the control of Scer\GAL4^G0431. The defect in memory after spaced training is still seen if the temperature sensitive Scer\GAL80^ts.αTub84B allele is used to restrict Scer\GAL4 expression to the adult stage.

(Chen et al., 2012)

Nmdar1^{dsRNA.Scer\UAS.cWa} expression in either the Scer\GAL4^GH146 or Scer\GAL4^VPN subset of neurons expressing TrpA1^{Scer\UAS.(B).cKa} causes an odorant-selective block in projection neuron-induced habituation. Thus, while 30 minutes of exposure to 29[o]C reduces the CO[[2]] avoidance of Scer\GAL4^VPN>TrpA1^{Scer\UAS.(B).cKa} flies, it has no effect on the CO[[2]] avoidance of Scer\GAL4^VPN/+; TrpA1^{Scer\UAS.(B).cKa}/+; Nmdar1^{dsRNA.Scer\UAS.cWa} flies. Similarly, the reduced ethyl butyrate avoidance of Scer\GAL4^GH146/+; TrpA1^{Scer\UAS.(B).cKa}/+ flies observed after heat exposure is not evident in Scer\GAL4^GH146/+; TrpA1^{Scer\UAS.(B).cKa}/Nmdar1^{dsRNA.Scer\UAS.cWa} flies.

(Sudhakaran et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Nmdar1^RNAi.UAS.cWa

Nmdar1^{dsRNA.Scer\UAS.cWa}

Nmdar1^{dsRNA.UAS.cWa}

dsNR1

(Chen et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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