FlyBase Allele Report: Dmel\park[UAS.cSa]

General Information

Symbol

Dmel\park^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Shiba-Fukushima

FlyBase ID

FBal0302842

Feature type

allele

Associated gene

Dmel\park

Associated Insertion(s)

Carried in Construct

P{UAS-park.S}

Also Known As

UAS-dParkin, UAS-Parkin, UAS-dParkin WT

Key Links

Transgenic product class

wild_type

(Shiba-Fukushima et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Shiba-Fukushima et al., 2014)

Mutagen

in vitro construct

(Shiba-Fukushima et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-park.S}

Cytology

Description

UASt regulatory sequences drive expression of wild type park.

(Shiba-Fukushima et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

park

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease 2

CEC

(Liu et al., 2022)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease 6

modeled by Pink1^B9

(Shiba-Fukushima et al., 2017)

exacerbates Parkinson's disease 6

modeled by Pink1⁵

(Liu et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^Mhc.PU

(Shiba-Fukushima et al., 2014)

abnormal neuroanatomy, with Scer\GAL4^ple.PU

(Shiba-Fukushima et al., 2014)

Phenotype Manifest In

dopaminergic neuron | progressive, with Scer\GAL4^ple.PU

(Shiba-Fukushima et al., 2014)

indirect flight muscle cell, with Scer\GAL4^Mhc.PU

(Shiba-Fukushima et al., 2014)

mitochondrion, with Scer\GAL4^Mhc.PU

(Shiba-Fukushima et al., 2014)

mitochondrion, with Scer\GAL4^ple.PU

(Shiba-Fukushima et al., 2014)

Detailed Description

Statement

Reference

Expression of park^Scer\UAS.cSa in indirect flight muscles under the control of Scer\GAL4^Mhc.PU shortens the mitochondria in the direction of the long axis compared with controls. ATP levels are similar to controls.

Expression of park^Scer\UAS.cSa under the control of Scer\GAL4^Mhc.PU improves the decline in climbing ability usually seen in older flies, although performance in 7-day old flies is reduced compared to controls.

Expression of park^Scer\UAS.cSa under the control of Scer\GAL4^ple.PU causes a reduction in the number of tubular mitochondria in the cell bodies of dopaminergic neurons in 5 day old flies compared to controls. The mitochondria usually observed outside of the cell body are not seen.

Flies expressing park^Scer\UAS.cSa under the control of Scer\GAL4^ple.PU do not exhibit reduced flying ability compared to controls.

Expression of park^Scer\UAS.cSa under the control of Scer\GAL4^ple.PU results in an age-dependent loss of dopaminergic neurons. Lifespan is unaffected.

(Shiba-Fukushima et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ple.PU, park^UAS.cSa has abnormal neuroanatomy phenotype, suppressible | partially by Pink1^B9

(Shiba-Fukushima et al., 2014)

Suppressor of

Statement

Reference

park^UAS.cSa/Scer\GAL4^Mhc.PU is a suppressor of visible phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

park^UAS.cSa/Scer\GAL4^Mhc.PU is a suppressor of short lived phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

park^UAS.cSa/Scer\GAL4^Mhc.PU is a suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Mhc.PU, park^UAS.cSa has indirect flight muscle cell phenotype, suppressible by Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Shiba-Fukushima et al., 2014)

Scer\GAL4^Mhc.PU, park^UAS.cSa has mitochondrion phenotype, suppressible by Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Shiba-Fukushima et al., 2014)

Scer\GAL4^ple.PU, park^UAS.cSa has dopaminergic neuron phenotype, suppressible | partially by Pink1^B9

(Shiba-Fukushima et al., 2014)

Scer\GAL4^ple.PU, park^UAS.cSa has mitochondrion phenotype, suppressible by Pink1^B9

(Shiba-Fukushima et al., 2014)

Suppressor of

Statement

Reference

Scer\GAL4^ple.PU/park^UAS.cSa is a suppressor of mitochondrion phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

park^UAS.cSa, Scer\GAL4^Mhc.PU is a suppressor of mitochondrion phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Shiba-Fukushima et al., 2014)

park^UAS.cSa/Scer\GAL4^Mhc.PU is a suppressor of indirect flight muscle cell phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

park^UAS.cSa/Scer\GAL4^Mhc.PU is a suppressor of mitochondrion phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

park^UAS.cSa/Scer\GAL4^Mhc.PU is a suppressor of wing phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

park^UAS.cSa/Scer\GAL4^Mhc.PU is a suppressor of thorax phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

Scer\GAL4^ple.PU/park^UAS.cSa is a suppressor of dopaminergic neuron phenotype of Pink1^B9

(Shiba-Fukushima et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The abnormally large, fused mitochondria observed in Pink1^B9 mutant flies are completely rescued when park^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Mhc.PU.

Expression of Pink1^{dsRNA.Scer\UAS} suppresses the reduction in mitochondria length seen when park^Scer\UAS.cSa is expressed in indirect flight muscles under the control of Scer\GAL4^Mhc.PU.

Expression of park^Scer\UAS.cSa under the control of Scer\GAL4^Mhc.PU suppresses the reduction in ATP levels seen in Pink1^B9 mutant flies. The reduction in climbing ability and wing and thorax defects are also rescued.

Expression of park^Scer\UAS.cSa suppresses the increase in mitochondrial length seen when Pink1^{dsRNA.Scer\UAS} is expressed under control of Scer\GAL4^Mhc.PU.

Expression of park^Scer\UAS.cSa under the control of Scer\GAL4^ple.PU in a Pink1^B9 mutant background rescues the mitochondrial aggregation and distribution defects seen with either mutation alone.

Pink1^B9 partially suppresses the dopaminergic neuron loss seen when park^Scer\UAS.cSa is expressed under the control of Scer\GAL4^ple.PU.

(Shiba-Fukushima et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^da.PU/park^UAS.cSa rescues park^Δ21/park¹

(Shiba-Fukushima et al., 2014)

park^UAS.cSa/Scer\GAL4^Mhc.PU rescues park^Δ21/park¹

(Shiba-Fukushima et al., 2014)

Comments

Expression of park^Scer\UAS.cSa under the control of Scer\GAL4^da.PU rescues the mitochondria phenotype seen in park^Δ21/park¹ mutant indirect flight muscles. The reduction in ATP levels is also rescued, and is improved to a level higher than that of controls.

Expression of park^Scer\UAS.cSa under the control of Scer\GAL4^Mhc.PU rescues the wing posture and thorax defects seen in park^Δ21/park¹ mutant flies.

(Shiba-Fukushima et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

park^Scer\UAS.cSa

park^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Shiba-Fukushima

Secondary FlyBase IDs

References (8)

Report Sections

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