Reference Report

Reference
Citation	Zhou, C., Huang, H., Kim, S.M., Lin, H., Meng, X., Han, K.A., Chiang, A.S., Wang, J.W., Jiao, R., Rao, Y. (2012). Molecular Genetic Analysis of Sexual Rejection: Roles of Octopamine and Its Receptor OAMB in Drosophila Courtship Conditioning. J. Neurosci. 32(41): 14281--14287. (Export to RIS)
FlyBase ID	FBrf0219612
Publication Type	Research paper
PubMed ID	23055498
PubMed Abstract	After Drosophila males are rejected by mated females, their subsequent courtship is inhibited even when encountering virgin females. Molecular mechanisms underlying courtship conditioning in the CNS are unclear. In this study, we find that tyramine β hydroxylase (TβH) mutant males unable to synthesize octopamine (OA) showed impaired courtship conditioning, which could be rescued by transgenic TβH expression in the CNS. Inactivation of octopaminergic neurons mimicked the TβH mutant phenotype. Transient activation of octopaminergic neurons in males not only decreased their courtship of virgin females, but also produced courtship conditioning. Single cell analysis revealed projection of octopaminergic neurons to the mushroom bodies. Deletion of the OAMB gene encoding an OA receptor expressed in the mushroom bodies disrupted courtship conditioning. Inactivation of neurons expressing OAMB also eliminated courtship conditioning. OAMB neurons responded robustly to male-specific pheromone cis-vaccenyl acetate in a dose-dependent manner. Our results indicate that OA plays an important role in courtship conditioning through its OAMB receptor expressed in a specific neuronal subset of the mushroom bodies.
DOI	10.1523/JNEUROSCI.0517-12.2012
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Publication Year	1981-
ISBN/ISSN	0270-6474 1529-2401
Data from Reference
Alleles (19)
	Avic\GFP^{GCaMP3.20xScer\UAS} Avic\GFP^{spGFP1-10.Scer\UAS.T:Hsap\CD4} Avic\GFP^{spGFP11.Ecol\lexAop.T:Hsap\CD4} Ecol\lexA^L5076 Ecol\lexA^L5251 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Oamb^Scer\UAS.cZa Oamb^unspecified Rnor\CD2^{Ecol\lexAop.T:Avic\GFP} Scer\GAL4^c739 Scer\GAL4^elav.PU Scer\GAL4^NP1131 Scer\GAL4^Oamb.KI Scer\GAL4^Tdc2.PC shi^1.Scer\UAS shi¹ Tbh^nM18 Tbh^Scer\UAS.cMa TrpA1^Scer\UAS.cKa
Constructs (12)
	P{20XUAS-GCaMP3} P{elav-GAL4.U} P{lexA} P{lexAop-CD4-spGFP11} P{lexAop-rCD2-GFP} P{Tdc2-GAL4.C} P{UAS-CD4-spGFP1-10} P{UAS-mCD8::GFP.L} P{UAS-Oamb.Z} P{UAS-shi^ts1.K} P{UAS-Tbh.M} P{UAS-TrpA1.K}
Genes (11)
	Avic\GFP brp Ecol\lexA Mmus\Cd8a Oamb Rnor\CD2 Scer\GAL4 shi Tbh Tdc2 TrpA1
Insertions (4)
	P{GawB}Hr39^c739 P{GawB}NP1131 P{lexA}L5076 P{lexA}L5251-LexA
Natural transposons (1)
	P-element
Transcripts (4)
	Ecol\lexA^L5076RA Ecol\lexA^L5251RA Scer\GAL4^Oamb.KIRA Scer\GAL4^Tdc2.PCRA