Dmel\P{lacW}EcR^k06210 Insertion

General Information
Symbol	Dmel\P{lacW}EcR^k06210	Species	D. melanogaster
Name		FlyBase ID	FBti0007240
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	CG14589, Ecol\lacZ, EcR	Viability / fertility
Causes allele(s)	CG14589^k06210, Ecol\lacZ^EcR-k06210, EcR^k06210	Stock availability	2 publicly available
LINE ID	l(2)k06210
Genomic Location
Chromosomal location	2R ( 42A11 )	Sequence location	2R:2,025,171..2,025,171 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:2,025,171..2,025,171 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	42A11 ( inferred by FlyBase from sequence location )
Cytological location (reported)	42A10-42A12 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ025786 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	CG14589 (BDGP Project Members, 1994-1999, Spradling et al., 1999, FlyBase Genome Annotators, 2004) Ecol\lacZ (BDGP Project Members, 1994-1999) EcR (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	CG14589^k06210 (BDGP Project Members, 1994-1999, Spradling et al., 1999, FlyBase Genome Annotators, 2004) Ecol\lacZ^EcR-k06210 (BDGP Project Members, 1994-1999) EcR^k06210 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Gates et al., 2004, Sedkov et al., 2003, D'Avino and Thummel, 2000, FlyBase Genome Annotators, 2004)

Lethality References lethal (D'Avino and Thummel, 2000) lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999) partially lethal - majority die \| pharate adult stage \| recessive (D'Avino and Thummel, 2000)
Sterility References
Phenotype Manifest In
	anterior crossvein \| ectopic (D'Avino and Thummel, 2000) leg \| pharate adult stage (Pentek et al., 2009) wing (D'Avino and Thummel, 2000) wing vein (D'Avino and Thummel, 2000) wing vein \| ectopic (D'Avino and Thummel, 2000) wing vein L3 \| ectopic (D'Avino and Thummel, 2000)
Detailed Description
Statement Reference Mutant pharate adults show a bent leg phenotype. (Pentek et al., 2009) No eye phenotype is seen in these mutants. (Sedkov et al., 2003) 2/3 of homozygotes die as pharate adults, while the remainder survive to adulthood. These adults die after about 1 week at 25^oC, but are fertile and can survive up to three weeks at 18^oC. These flies also exhibit cell adhesion and morphogenetic defects. 1/4 display venation defects, 1/3 display wing blisters. These blisters are usually small and centrally located, although blistering of the whole wing (a balloon wing) can occasionally be seen. The most frequent venation defects include a small, extra vein that originates from the third longitudinal vein, an additional anterior crossvein, and a "delta" thickening at the intersection between the posterior crossvein and the fourth longitudinal vein. EcR^k06210/EcR^M554fs flies are fully lethal. FlyBase curator comment: Since the "k06210" insertion lies within EcR and the EcR-intronic CG14589 it is not clear which disruption might be responsible for the lethality. (D'Avino and Thummel, 2000) 21% of EcR^k06210/EcR²¹⁴ flies survive to adulthood. (D'Avino and Thummel, 2000) FlyBase curator comment: Since the "k06210" insertion lies within EcR and the EcR-intronic CG14589 it is not clear which disruption might be responsible for the lethality. (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k06210 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10614 y¹ w^67c23; P{lacW}EcR^k06210 CG14589^k06210/CyO
Kyoto	102456 y¹ w^67c23 ; P{lacW}EcR^k06210 / CyO
Linkouts
Comments
	Location 2R:1645054-1645055 confirmed by FlyBase alignment of dbGSS accession AQ025786 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}EcR^k06210 (FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 13 )
Research paper	Pentek et al., 2009, genesis 47(4): 261--273 Follistatin preferentially antagonizes activin rather than BMP signaling in Drosophila. [FBrf0207743] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Gates et al., 2004, Development 131(1): 25--36 rigor mortis encodes a novel nuclear receptor interacting protein required for ecdysone signaling during Drosophila larval development. [FBrf0167527] D'Avino and Thummel, 2000, Dev. Biol. 220(2): 211--224 The ecdysone regulatory pathway controls wing morphogenesis and integrin expression during Drosophila metamorphosis. [FBrf0127048] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase Genome Annotators, 2004, Changes affecting gene model number or type in release 3.2 of the annotated D.melanogaster genome. Changes affecting gene model number or type in release 3.2 of the annotated D.melanogaster genome. [FBrf0166453] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]
Letter	Sedkov et al., 2003, Nature 426(6962): 78--83 Methylation of lysine 4 of histone H3 in ecdysone-dependent development of Drosophila. [FBrf0173071]

Dmel\P{lacW}EcRk06210 Insertion

Dmel\P{lacW}EcR^k06210 Insertion