Dmel\P{lacW}dom^k08108 Insertion

General Information
Symbol	Dmel\P{lacW}dom^k08108	Species	D. melanogaster
Name		FlyBase ID	FBti0009608
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	dom, Ecol\lacZ	Viability / fertility
Causes allele(s)	dom^k08108, Ecol\lacZ^dom-k08108	Stock availability	2 publicly available
LINE ID	l(2)k08108
Genomic Location
Chromosomal location	2R ( 57D11 )	Sequence location	2R:17,211,119..17,211,119 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:17,211,119..17,211,119 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	57D11 ( inferred by FlyBase from sequence location )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence	AQ034011 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	dom (BDGP Project Members, 1994-1999, Chen et al., 2008) Ecol\lacZ (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	dom^k08108 (BDGP Project Members, 1994-1999, Ruhf et al., 2001, Braun et al., 1997, Roch et al., 1998, Kurucz et al., 2003, Spradling et al., 1999, Chen et al., 2008) Ecol\lacZ^dom-k08108 (BDGP Project Members, 1994-1999)

Lethality References cell lethal \| somatic clone (Ruhf et al., 2001) lethal \| pupal stage (Ragab, 2006) lethal \| pupal stage \| recessive (Braun et al., 1997, Ruhf et al., 2001) lethal \| recessive (Spradling et al., 1999, Roch et al., 1998)
Sterility References female sterile \| germline clone \| recessive (Ruhf et al., 2001)
Phenotype Manifest In
	embryonic/larval hemocyte (Braun et al., 1998) embryonic/larval lymph gland (Ragab, 2006, Braun et al., 1997, Ruhf et al., 2001) follicle stem cell \| somatic clone (Xi and Xie, 2005) hemocyte (Ragab, 2006, Braun et al., 1997, Ruhf et al., 2001) imaginal disc (Roch et al., 1998, Braun et al., 1997, Ruhf et al., 2001) imaginal ring (Braun et al., 1997) midgut imaginal island (Braun et al., 1997) neuroblast (Ruhf et al., 2001) plasmatocyte (Braun et al., 1998) prohemocyte (Braun et al., 1997)
Detailed Description
Statement Reference Heterozygosity for dom[k08108] increases the number of wing margin nicks seen in mam[g2.1] heterozygotes. Heterozygosity for dom[k08108] further reduces the width of N[nd-1] wings. Wing vein L4 is shortened in N[Ax-E2] mutants; heterozygosity for dom[k08108] slightly increases the length of L4. (Gause et al., 2006) Df(2R)ED3921 fails to suppress the melanized lymph gland and lack of hemocyte phenotype of dom^k08108 mutants . (Ragab, 2006) dom^k08108 mutants exhibit melanized lymph glands and a lack of hemocytes. (Ragab, 2006) dom^k08108 homozygous female germ-line stem cells are lost from their stem cell niche at the same rate as wild-type female germ-line stem cells. However, 91% of dom³ homozygous clone follicle stem cells are lost from their niche within 17 days, compared to only 37.5% of cells in wild-type control clones. This is not accompanied by any increase in in apoptosis of these cells. (Xi and Xie, 2005) Lethality acts in the early pupal stages. Lymph glands are black, no hemocytes and reduced neuroblast region in the larval brain. Mititic defects are not evident in the neuroblasts. Females with germline clones do not lay eggs. (Ruhf et al., 2001) Homozygous third instar larvae have a striking absence of circulating hemocytes; only 0 to 10 oversized cells are seen per larva, in contrast to the wild-type number of 1000-3000 circulating hemocytes. In addition, the number of sessile plasmatocytes is significantly reduced compared to wild-type. Homozygous larvae contain large numbers of microorganisms which can be seen on the epithelium lining the integument and on muscles. The melanisation in response to pricking with a needle is reduced in intensity compared to wild-type larvae. Melanisation plaques appear on the surface of homozygous larvae after natural infection by spores of B.bassiana, as are seen in wild-type larvae. There is not a significant difference in the survival rate between larvae pricked with a sterile needle or a needle coated with Gram-positive or Gram-negative bacteria, however, infection with fungal spores induces over 60% lethality in 48 hours in homozygous larvae. (Braun et al., 1998) Tl[8]/+ ; dom[k08108], cact[7] dom[k08108] or hop[Tum] ; dom[k08108] double mutant larvae contain melanotic masses, although their frequency is markedly reduced compared to Tl[8]/+, cact[7] or hop[Tum] single mutant larvae and the masses are devoid of hemocytes. dom[k08108] imd[1] and dom[k08108] Bc[1] double mutant larvae show a dramatically compromised survival after injury (whether it is a clean injury or an injury combined with infection). (Braun et al., 1998) Lethality occurs during third instar larval or pupal stages. Mutants exhibit visible disc abnormalities: very small or no discs. Clones cannot be induced in wild type discs. In tergites, clones with normal size and but lower than expected frequency are recovered. Clones cannot be recovered in the wing disc. (Roch et al., 1998) Homozygous larvae are totally devoid of circulating hemocytes. In favourable conditions these larvae have a prolonged third instar, up to 10 days at 20^oC. They show melanized lymph glands. During third instar the blackening invades the whole lobe, extending to the posterior lobes. Melanized lymph glands eventually detach from the dorsal vessel. Ultrastructural analysis reveals that the prohemocytes in the hematopoetic organ are larger than wild type. Lymph glands are filled with necrotic, melanized cells and cells packed with heterogeneous inclusions indicative of strong resorptive processes. Mutant glands are devoid of differentiating prohemocytes. The embryonic hemocytes are unaffected, but by first instar hemocytes are reduced in number. Mutants are also devoid of imaginal discs, imaginal rings and histoblasts in the gut. The size of the brain is significantly reduced. The domain of brain neuroblasts in the optic lobes is reduced. Residual imaginal disc cells exist in small clusters. (Braun et al., 1997)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k08108 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10767 y¹ w^67c23; P{lacW}dom^k08108/CyO
Kyoto	111237 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}dom^k08108 /CyO y⁺
Linkouts
Comments
	Location 2R:16831196-16831197 confirmed by FlyBase alignment of dbGSS accession AQ034011 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}dom^k08108 (FlyBase, 2005, FlyBase, 1992-, Chen et al., 2008, ) P{lacW}domino^k08108 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{lacW}l(2)k08108^k08108 (BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0006651
References ( 17 )
Research paper	Chen et al., 2008, Genome Res. 18(1): 123--136 Identification of novel modulators of mitochondrial function by a genome-wide RNAi screen in Drosophila melanogaster. [FBrf0202125] Gause et al., 2006, Mol. Cell. Biol. 26(6): 2347--2359 Nipped-A, the Tra1/TRRAP subunit of the Drosophila SAGA and Tip60 complexes, has multiple roles in Notch signaling during wing development. [FBrf0191191] Xi and Xie, 2005, Science 310(5753): 1487--1489 Stem cell self-renewal controlled by chromatin remodeling factors. [FBrf0190619] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Kurucz et al., 2003, Proc. Natl. Acad. Sci. U.S.A. 100(5): 2622--2627 Hemese, a hemocyte-specific transmembrane protein, affects the cellular immune response in Drosophila. [FBrf0159287] Ruhf et al., 2001, Development 128(8): 1429--1441 The domino gene of Drosophila encodes novel members of the SWI2/SNF2 family of DNA-dependent ATPases, which contribute to the silencing of homeotic genes. [FBrf0134570] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Braun et al., 1998, Proc. Natl. Acad. Sci. U.S.A. 95(24): 14337--14342 Analysis of the Drosophila host defense in domino mutant larvae, which are devoid of hemocytes. [FBrf0105781] Roch et al., 1998, Mol. Gen. Genet. 257(2): 103--112 Screening of larval/pupal P-element induced lethals on the second chromosome in Drosophila melanogaster: clonal analysis and morphology of imaginal discs. [FBrf0100624] Braun et al., 1997, Genetics 147(2): 623--634 Drosophila immunity: analysis of larval hemocytes by P-element-mediated enhancer trap. [FBrf0098749] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Ragab, 2006, Genetics 172(2): Supplemental data. [FBrf0191785]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}domk08108 Insertion

Dmel\P{lacW}dom^k08108 Insertion