Dmel\P{EP}rho^EP3704 Insertion

General Information
Symbol	Dmel\P{EP}rho^EP3704	Species	D. melanogaster
Name		FlyBase ID	FBti0011785
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	rho	Viability / fertility
Causes allele(s)	rho^EP3704	Stock availability	2 publicly available
LINE ID	EP(3)3704
Genomic Location
Chromosomal location	3L ( 62A2 )	Sequence location	3L:1,463,695..1,463,695 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:1,463,695..1,463,695 [+] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	62A2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	62A1-62A2 (in situ hybridization reported) (BDGP Project Members, 1994-1999) 62A3-62A3 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ254746 (FlyBase, 1992-)
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	rho (BDGP Project Members, 2000-, Bidet et al., 2001)
Alleles and Phenotypes
Causes alleles	rho^EP3704 (Tseng and Hariharan, 2002, McGovern et al., 2003, Bidet, 2003, Liu et al., 2006)

Lethality References lethal (Tseng and Hariharan, 2002) viable (McGovern et al., 2003)
Sterility References
Phenotype Manifest In
	embryonic pericardial cell (Bidet, 2003) eye (Tseng and Hariharan, 2002) segment border muscle (Bidet, 2003) ventral nerve cord commissure (McGovern et al., 2003)
Detailed Description
Statement Reference Embryos expressing rho^EP3704 under the control of Scer\GAL4^how-24B show a massive overproduction of lateral adult muscle precursors at the expense of the segmental border muscle fibre. Embryos expressing rho^EP3704 under the control of Scer\GAL4^how-24B show an increased number of tin-expressing and tin/eve-expressing pericardial cells (some of which are intermingled with dorsal muscle cells) accompanied by a reduced number of tin/lbe-expressing pericardial cells. (Bidet, 2003) ventral nerve cord commissures in stage 17 rho^EP3704; Scer\GAL4^sca-4512 embryos are fused and reduced. (McGovern et al., 2003) Expression of rho^EP3704 under the control of Scer\GAL4^ey.PH results in rough eyes. Flies expressing rho^EP3704 under the control of Scer\GAL4^hs.2sev have glassy eyes. (Tseng and Hariharan, 2002)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(3)3704 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	17276 w¹¹¹⁸; P{EP}rho^EP3704
Kyoto	109018 w^*; P{EP}rho^EP3704 / TM6B, Tb¹
Linkouts
Comments
	Location 3L:1447227-1447228 confirmed by FlyBase alignment of dbGSS accession AQ254746 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP3704 (Bidet et al., 2001) EP(3)3704 (Bidet, 2003, McGovern et al., 2003, FlyBase, 1992-, Liu et al., 2006) P{EP}EP3704 (FlyBase, 1992-) P{EP}rho^EP3704 (FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 13 )
Research paper	Liu et al., 2006, Dev. Biol. 290(2): 373--385 Hedgehog and RAS pathways cooperate in the anterior-posterior specification and positioning of cardiac progenitor cells. [FBrf0190138] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Bidet, 2003, Mech. Dev. 120(9): 991--1007 Modifiers of muscle and heart cell fate specification identified by gain-of-function screen in Drosophila. [FBrf0173131] McGovern et al., 2003, Mech. Dev. 120(10): 1193--1207 A targeted gain of function screen in the embryonic CNS of Drosophila. [FBrf0167840] Tseng and Hariharan, 2002, Genetics 162(1): 229--243 An overexpression screen in Drosophila for genes that restrict growth or cell-cycle progression in the developing eye. [FBrf0152035] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Rorth, 1996, Proc. Natl. Acad. Sci. USA 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Abstract	Bidet et al., 2001, A. Dros. Res. Conf. 42: 670A Identification and analysis of EP lines involved in heart and muscle formation: I) New role of previously described genes. [FBrf0133802]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{EP}rhoEP3704 Insertion

Dmel\P{EP}rho^EP3704 Insertion