FlyBase Allele Report: Dmel\rho[EP3704]

General Information

Symbol

Dmel\rho^EP3704

Species

D. melanogaster

Name

FlyBase ID

FBal0140963

Feature type

allele

Associated gene

Dmel\rho

Associated Insertion(s)

P{EP}rho^EP3704

Carried in Construct

Also Known As

EP(3)3704

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Bidet et al., 2003, McGovern et al., 2003, Tseng and Hariharan, 2002)

Progenitor genotype

Associated Insertion(s)

P{EP}rho^EP3704

Cytology

Description

Capable of over-expressing rho.

(Maybeck and Röper, 2009)

P{EP} insertion 458bp upstream of the rho translation initiation codon. The P{EP} element is oriented such that expression from the element results in overexpression of rho.

(Bidet et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:1,463,695..1,463,695 [+]

(Schwarz et al., 2018, Sanhueza et al., 2015, Maybeck and Röper, 2009, Liu et al., 2006, Bidet et al., 2003, McGovern et al., 2003, Tseng and Hariharan, 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

viable, with Scer\GAL4^sca-4512

(McGovern et al., 2003)

visible, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^hs.2sev

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^sd.PU

(Rougeot et al., 2013)

visible | adult stage, with Scer\GAL4^sd.PU

(Mouchel-Vielh et al., 2011)

Phenotype Manifest In

abdominal dorsal adult muscle precursor cell, with Scer\GAL4^69B

(Figeac et al., 2010)

abdominal dorsal adult muscle precursor cell, with Scer\GAL4^how-24B

(Figeac et al., 2010)

abdominal dorsal adult muscle precursor cell, with Scer\GAL4^kirre.PR

(Figeac et al., 2010)

abdominal dorsolateral adult muscle precursor cell, with Scer\GAL4^69B

(Figeac et al., 2010)

abdominal dorsolateral adult muscle precursor cell, with Scer\GAL4^how-24B

(Figeac et al., 2010)

abdominal dorsolateral adult muscle precursor cell, with Scer\GAL4^kirre.PR

(Figeac et al., 2010)

abdominal lateral adult muscle precursor cell, with Scer\GAL4^69B

(Figeac et al., 2010)

abdominal lateral adult muscle precursor cell, with Scer\GAL4^how-24B

(Figeac et al., 2010)

abdominal lateral adult muscle precursor cell, with Scer\GAL4^kirre.PR

(Figeac et al., 2010)

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

(Maybeck and Röper, 2009)

embryonic pericardial cell, with Scer\GAL4^how-24B

(Bidet et al., 2003)

eye, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

eye, with Scer\GAL4^hs.2sev

(Tseng and Hariharan, 2002)

larval ventral nerve cord commissure, with Scer\GAL4^sca-4512

(McGovern et al., 2003)

segment border muscle cell, with Scer\GAL4^how-24B

(Bidet et al., 2003)

wing, with Scer\GAL4^sd.PU

(Mouchel-Vielh et al., 2011)

wing margin, with Scer\GAL4^sd.PU

(Rougeot et al., 2013)

wing vein | ectopic, with Scer\GAL4^sd.PU

(Rougeot et al., 2013, Mouchel-Vielh et al., 2011)

Detailed Description

Statement

Reference

Expression of rho^EP3704 under the control of Scer\GAL4^sd.PU results in ectopic wing veins and wing margin defects.

(Rougeot et al., 2013)

Flies carrying rho^EP3704/+ alone do not exhibit any ectopic wing vein or blistered wing phenotypes.

Expression of rho^EP3704 under the control of Scer\GAL4^sd.PU leads to induction of ectopic wing veins in most of the flies while it leads to formation of wing blisters in a few flies.

(Mouchel-Vielh et al., 2011)

Embryos expressing rho^EP3704 under the control of either Scer\GAL4^how-24B, Scer\GAL4^kirre.PR or Scer\GAL4^69B show an increase in the number of dorsal, dorsolateral and lateral adult muscle precursor cells compared to wild type. The number of ventral adult muscle precursor cells is unaffected in these embryos.

(Figeac et al., 2010)

Expression via Scer\GAL4^fkh.PH results in a very strongly penetrant aberrant duct morphogenesis phenotype in the embryonic salivary gland. Glands are positioned too anteriorly with no obvious distinction in shape between duct and secretory cells and no common duct-like structure formed at stage 15. At earlier stages during the invagination process, aberrantly shaped lumena are observed, but most prominently a large bulge of eyg-positive ectopic cells seem to arise between the already invaginated secretory gland portions at the position where the individual ducts would normally form.

(Maybeck and Röper, 2009)

Embryos expressing rho^EP3704 under the control of Scer\GAL4^how-24B show a massive overproduction of lateral adult muscle precursors at the expense of the segmental border muscle fibre. Embryos expressing rho^EP3704 under the control of Scer\GAL4^how-24B show an increased number of tin-expressing and tin/eve-expressing pericardial cells (some of which are intermingled with dorsal muscle cells) accompanied by a reduced number of tin/lbe-expressing pericardial cells.

(Bidet et al., 2003)

ventral nerve cord commissures in stage 17 rho^EP3704; Scer\GAL4^sca-4512 embryos are fused and reduced.

(McGovern et al., 2003)

Expression of rho^EP3704 under the control of Scer\GAL4^ey.PH results in rough eyes. Flies expressing rho^EP3704 under the control of Scer\GAL4^hs.2sev have glassy eyes.

(Tseng and Hariharan, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^sd.PU, rho^EP3704 has visible | adult stage phenotype, enhanceable by corto[+]/corto⁴²⁰

(Mouchel-Vielh et al., 2011)

Scer\GAL4^sd.PU, rho^EP3704 has visible | adult stage phenotype, enhanceable by corto^07128b/corto[+]

(Mouchel-Vielh et al., 2011)

Suppressor of

Statement

Reference

rho^EP3704, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^sd.PU, rho^EP3704 has wing phenotype, enhanceable by corto[+]/corto⁴²⁰

(Mouchel-Vielh et al., 2011)

Scer\GAL4^sd.PU, rho^EP3704 has wing phenotype, enhanceable by corto^07128b/corto[+]

(Mouchel-Vielh et al., 2011)

Suppressor of

Statement

Reference

rho^EP3704, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Either corto⁴²⁰/+ or corto^07128b/+ enhances the blistered wing phenotype induced by expression of rho^EP3704 under the control of Scer\GAL4^sd.PU.

(Mouchel-Vielh et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

17276

w¹¹¹⁸; P{EP}rho^EP3704

Kyoto

109018

w^*; P{EP}rho^EP3704 / TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

EP3704

(Figeac et al., 2010)

rho^EP3704

(Rougeot et al., 2013, Mouchel-Vielh et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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