Dmel\P{SUPor-P}Drp1^KG03815 Insertion

General Information
Symbol	Dmel\P{SUPor-P}Drp1^KG03815	Species	D. melanogaster
Name		FlyBase ID	FBti0021561
Feature type	transposable_element_insertion_site
Description
Inserted element	P{SUPor-P}	Expression data
Affected gene(s)	Drp1	Viability / fertility
Causes allele(s)	Drp1^KG03815	Stock availability	2 publicly available
LINE ID	KG03815
Genomic Location
Chromosomal location	2L ( 23A1 )	Sequence location	2L:2,584,872..2,584,872 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:2,584,872..2,584,872 [+] (Gene Disruption Project members, 2001-)
Orientation
Cytological location (computed by FlyBase)	23A1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	23A1 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	BH740812 (Gene Disruption Project members, 2001-, FlyBase, 1992-)
Inserted Element
Construct	P{SUPor-P}
Location-dependent role
Size	11.467Kb
Associated alleles	gypsy\su(Hw)BR⁴³⁰ w^BR.E.BR y^+mDint2
Molecular map
Affected Gene(s)
Insertion may affect gene	Drp1 (Gene Disruption Project members, 2001-, Verstreken et al., 2005)
Alleles and Phenotypes
Causes alleles	Drp1^KG03815 (Gene Disruption Project members, 2001-, Ly, 2005.3.2, Verstreken et al., 2005)

Lethality References
Sterility References
Phenotype Manifest In
	autophagic vacuole & larval fat body \| somatic clone \| cell autonomous (Arsham and Neufeld, 2009) lysosome & larval fat body \| somatic clone \| cell autonomous (Arsham and Neufeld, 2009) mitochondrion & primary spermatocyte \| germ-line clone (Aldridge et al., 2007)
Detailed Description
Statement Reference Drp1[KG03815] clones in fat bodies from well-fed early third instar larvae accumulate lysosomes, as indicated by elevated LysoTracker staining, and show redistribution of the Atg8a product into autophagosomes. (Arsham and Neufeld, 2009) Primary spermatocytes in homozygous germline clones induced in males contain abnormally clustered mitochondria. 52% of early round spermatids derived from these spermatocytes retain mitochondrial material and in all cases the mitochondria appear not to have separated properly during meiosis, remaining connected and appearing to pass through the cytoplasmic bridges between spermatids. The remaining 48% of the mutant spermatids have little or no mitochondrial material. At later stages of flagellar elongation, the mutant spermatids have defects in unfurling and elongation of mitochondria, with the spermatids containing spaghetti-like interconnected masses of mitochondria that fail to elongate properly and are associated with multiple nuclei. The ratio of the observed mitochondrial masses to nuclei is 8:34, consistent with each mass typically representing the mitochondrial material from all four meiotic products of a single spermatocyte. (Aldridge et al., 2007)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	KG03815 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	13510 y¹; P{SUPor-P}Drp1^KG03815/CyO; ry⁵⁰⁶
Kyoto	111549 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{SUPor-P}Drp1^KG03815 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	Location 2L:2584872-2584873 confirmed by FlyBase alignment of dbGSS accession BH740812 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	KG03815 (Gene Disruption Project members, 2001-, FlyBase, 1992-, Verstreken et al., 2005) P{SUPor-P}CG3210^KG03815 (FlyBase, 1992-) P{SUPor-P}Drp1^KG03815 (FlyBase, 2005, FlyBase, 1992-) P{SUPor-P}noodle^KG03815 (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 8 )
Research paper	Arsham and Neufeld, 2009, PLoS ONE 4(6): e6068 A genetic screen in Drosophila reveals novel cytoprotective functions of the autophagy-lysosome pathway. [FBrf0208155] Aldridge et al., 2007, Fly 1(1): 38--46 Roles for Drp1, a dynamin-related protein, and milton, a kinesin-associated protein, in mitochondrial segregation, unfurling, and elongation during Drosophila spermatogenesis. [FBrf0200064] Verstreken et al., 2005, Neuron 47(3): 365--378 Synaptic mitochondria are critical for mobilization of reserve pool vesicles at Drosophila neuromuscular junctions. [FBrf0188182] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase	Ly, 2005.3.2, Helping FlyBase: ADRC-50399. Helping FlyBase: ADRC-50399. [FBrf0183137]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\P{SUPor-P}Drp1KG03815 Insertion

Dmel\P{SUPor-P}Drp1^KG03815 Insertion